{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T15:51:05Z","timestamp":1769183465861,"version":"3.49.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":204,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hospital Universitario-Centro Dermatol\u00f3gico Federico Lleras Acosta E.S.E, DC, Colombia","award":["4000.16.6AC"],"award-info":[{"award-number":["4000.16.6AC"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Introduction<\/jats:italic>. Mutations in the BRAF, NRAS, and C\u2010KIT genes have been associated with the histopathological characteristics of melanoma. Likewise, the incidence of each of these subtypes changes according to the geographical origin of the population analyzed. <jats:italic>Objective<\/jats:italic>. To determine the mutation frequency in exons 11 and 15 of the BRAF gene, exons 1 and 2 of the NRAS gene, and exons 11, 13, and 17 of the C\u2010KIT gene and to relate it with histological subtypes in patients from a region with high levels of ultraviolet radiation. <jats:italic>Methodology<\/jats:italic>. The clinicopathological characteristics of 54 cutaneous melanoma samples were analyzed. Mutation analysis was performed via qPCR on paraffin\u2010embedded tumor tissue samples using probes specific for the V600E mutation. Amplification of exons 11 and 15 of the BRAF gene, exons 1 and 2 of the NRAS gene, and exons 11, 13, and 17 of the C\u2010KIT gene was performed for subsequent sequencing using the Sanger method. <jats:italic>Result<\/jats:italic>. The most frequent histological subtype in the analyzed sample was lentigo maligna\/lentigo maligna melanoma (52%) followed by acral lentiginous melanoma (20%). The BRAF\u2010V600 variant was the most frequent (63.6%). The most frequent (54%) mutation in NRAS was p.Lys5\u2217. In the C\u2010KIT gene, only the Val560Ala mutation was found. <jats:italic>Conclusion<\/jats:italic>. Differences in histological subtypes and mutations in the BRAF, NRAS, and C\u2010KIT genes were found in the analyzed population. This indicates that environmental and genetic factors significantly influence the introduction of the disease in this geographic region.<\/jats:p>","DOI":"10.1155\/2020\/2046947","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T23:30:49Z","timestamp":1595547049000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Mutations in the BRAF, NRAS, and C\u2010KIT Genes of Patients Diagnosed with Melanoma in Colombia Population"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6155-3771","authenticated-orcid":false,"given":"Luz D.","family":"Guti\u00e9rrez-Casta\u00f1eda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio","family":"Gamboa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Nova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Pulido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose D.","family":"Tovar-Parra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21387"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.31551"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.suc.2014.07.013"},{"key":"e_1_2_10_4_2","unstructured":"International Agency for Research on Cancer Estimated number of incident cases of melanoma https:\/\/gco.iarc.fr\/today\/data\/factsheets\/populations\/170-colombia-fact-sheets.pdf."},{"key":"e_1_2_10_5_2","unstructured":"Cancer Facts Sheets: Melanoma of the Skin https:\/\/gco.iarc.fr\/today\/fact-sheets-cancers."},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30638"},{"key":"e_1_2_10_7_2","first-page":"114","article-title":"Genetics of melanoma susceptibility","volume":"13","author":"Ghiorzo P.","year":"2003","journal-title":"Forum (Genoa, Italy)"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.41.2452"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.12822"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10185.x"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-007-0524-2"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.14670\/HH-24.643"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.4238\/2014.January.22.8"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2007.12.003"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-18-1795"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2017.43"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.26724"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.186"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2015.39"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.044"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.51643\/22562915.347"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.rpd.a033230"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.01.035"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0123-9015(13)70010-6"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0124-00642007000400012"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/515S110a"},{"key":"e_1_2_10_27_2","first-page":"1005","article-title":"Melanoma: epidemiology, risk factors, pathogenesis, diagnosis and classification","volume":"28","author":"Rastrelli M.","year":"2014","journal-title":"In Vivo"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0123-9015(13)70013-1"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2004.10.032"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188602"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2015.02.1113"},{"key":"e_1_2_10_32_2","volume-title":"Incidencia de la mutaci\u00f3n del gen braf en pacientes con melanoma. 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