{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:22:57Z","timestamp":1788322977667,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,4,27]],"date-time":"2020-04-27T00:00:00Z","timestamp":1587945600000},"content-version":"vor","delay-in-days":117,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002822","name":"Central South University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002822","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. This study is aimed at investigating the efficacy of CTLA4\u2010Ig abatacept in normalizing proteinuria and its possible mechanism in adriamycin\u2010induced nephropathy (AIN) rats. <jats:italic>Methods<\/jats:italic>. A total of 32 healthy male Sprague\u2010Dawley rats were randomly divided into a normal group, an AIN group, an abatacept group, and a prednisone group. Adriamycin (6.5\u2009mg\/kg) was injected once via the tail vein of rats to induce nephrotic syndrome. After adriamycin treatment, the abatacept group rats were given abatacept (0.5\u2009mg\/kg) once by intraperitoneal injection on day 14. In addition, the prednisone group rats were given prednisone (12.5\u2009mg\/kg) daily consecutively by gavage from day 14 to day 21. Blood, urine, and kidney tissue specimens were collected when sacrificed on day 21. The 24\u2010hour urinary protein, serum albumin, cholesterol, creatinine, and urea nitrogen were then detected. An enzyme\u2010linked immunosorbent assay was used to determine the level of urine CD80 and serum IL\u201017. Flow cytometry was used to investigate the prevalence of circulating Treg. Hematoxylin\u2010eosin staining and electron microscopy were used for a renal histological study. Immunofluorescence staining was performed to confirm the CD80 expression of renal tissue. <jats:italic>Results<\/jats:italic>. The 24\u2010hour urinary protein of the abatacept group was significantly lower than that of the prednisone group and the AIN group. The level of urine CD80 of the abatacept group was significantly lower than that of the AIN group. Compared with the AIN group and the prednisone group, the circulating Treg prevalence of the abatacept group was significantly higher, while the level of serum IL\u201017 was lower. A negative kidney staining of CD80 expression was demonstrated in each group in this study. The 24\u2010hour urinary protein had a negative correlation with the circulating Treg prevalence and Treg\/IL\u201017 and a positive correlation with the urine CD80 and serum IL\u201017. Urinary CD80 had a positive correlation with serum IL\u201017 and no correlation with the circulating Treg prevalence. <jats:italic>Conclusions<\/jats:italic>. CTLA4\u2010Ig abatacept can reduce proteinuria of adriamycin\u2010induced nephropathy rats, possibly at least partially as a result of regulating circulating Treg\/IL\u201017. CTLA4\u2010Ig abatacept could be a promising regimen for idiopathic nephrotic syndrome.<\/jats:p>","DOI":"10.1155\/2020\/2347827","type":"journal-article","created":{"date-parts":[[2020,4,27]],"date-time":"2020-04-27T23:31:33Z","timestamp":1588030293000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["CTLA4\u2010Ig Abatacept Ameliorates Proteinuria by Regulating Circulating Treg\/IL\u201017 in Adriamycin\u2010Induced Nephropathy 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