{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T19:21:02Z","timestamp":1769973662661,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T00:00:00Z","timestamp":1585872000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670770"],"award-info":[{"award-number":["81670770"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300674"],"award-info":[{"award-number":["81300674"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700733"],"award-info":[{"award-number":["81700733"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772049"],"award-info":[{"award-number":["81772049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670770"],"award-info":[{"award-number":["81670770"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300674"],"award-info":[{"award-number":["81300674"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700733"],"award-info":[{"award-number":["81700733"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772049"],"award-info":[{"award-number":["81772049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670770"],"award-info":[{"award-number":["81670770"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300674"],"award-info":[{"award-number":["81300674"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700733"],"award-info":[{"award-number":["81700733"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772049"],"award-info":[{"award-number":["81772049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670770"],"award-info":[{"award-number":["81670770"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300674"],"award-info":[{"award-number":["81300674"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700733"],"award-info":[{"award-number":["81700733"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772049"],"award-info":[{"award-number":["81772049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2020,4,3]]},"abstract":"<jats:p>Diabetic hearts are more susceptible to myocardial ischemia\/reperfusion (I\/R) injury and less sensitive to ischemic postconditioning (IPostC), but the underlying mechanisms remain unclear. PKC<jats:italic>\u03b2<\/jats:italic>2 is preferentially overactivated in diabetic myocardium, in which autophagy status is abnormal. This study determined whether hyperglycemia-induced PKC<jats:italic>\u03b2<\/jats:italic>2 activation resulted in autophagy abnormality and compromised IPostC cardioprotection in diabetes. We found that diabetic rats showed higher cardiac PKC<jats:italic>\u03b2<\/jats:italic>2 activation and lower autophagy than control at baseline. However, myocardial I\/R further increased PKC<jats:italic>\u03b2<\/jats:italic>2 activation and promoted autophagy status in diabetic rats. IPostC significantly attenuated postischemic infarct size and CK-MB, accompanied with decreased PKC<jats:italic>\u03b2<\/jats:italic>2 activation and autophagy in control but not in diabetic rats. Pretreatment with CGP53353, a selective inhibitor of PKC<jats:italic>\u03b2<\/jats:italic>2, attenuated myocardial I\/R-induced infarction and autophagy and restored IPostC-mediated cardioprotection in diabetes. Similarly, CGP53353 could restore hypoxic postconditioning (HPostC) protection against hypoxia reoxygenation- (HR-) induced injury evidenced by decreased LDH release and JC-1 monomeric cells and increased cell viability. These beneficial effects of CGP53353 were reversed by autophagy inducer rapamycin, but could be mimicked by autophagy inhibitor 3-MA. It is concluded that selective inhibition of PKC<jats:italic>\u03b2<\/jats:italic>2 could attenuate myocardial I\/R injury and restore IPostC-mediated cardioprotection possibly through modulating autophagy in diabetes.<\/jats:p>","DOI":"10.1155\/2020\/2408240","type":"journal-article","created":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T23:30:35Z","timestamp":1585956635000},"page":"1-11","source":"Crossref","is-referenced-by-count":10,"title":["Selective Inhibition of PKC<i>\u03b2<\/i>2 Restores Ischemic Postconditioning-Mediated Cardioprotection by Modulating Autophagy in Diabetic Rats"],"prefix":"10.1155","volume":"2020","author":[{"given":"Yafeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0779-1226","authenticated-orcid":true,"given":"Wating","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengnan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6660-5123","authenticated-orcid":true,"given":"Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5807-9554","authenticated-orcid":true,"given":"Zhong-yuan","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7002-5524","authenticated-orcid":true,"given":"Zhengyuan","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The University of Hong Kong, Hong Kong SAR, China"},{"name":"Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0093-3068","authenticated-orcid":true,"given":"Shaoqing","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology, Renmin Hospital of Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.307937"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI62874"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-016-0588-8"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s12576-015-0365-8"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.108.765750"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1042\/CS20140789"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1042\/bj3320281"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2019.109417"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S105482"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1042\/CS20110176"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.301074"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20160445"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.4161\/auto.25239"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-018-1477-4"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2018100983"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109464"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2019.09.001"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.113677"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116748"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1042\/CS20170052"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9502895"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.2337\/db12-1391"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1042\/CS20150496"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.111.623991"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9743280"},{"issue":"9","key":"26","first-page":"15801","volume":"8","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00600.2004"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.314500"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/eht090"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60916-7"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/564902"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2015.045"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.12.020"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01346.2007"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2007.04.016"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.08.029"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600698R"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-016-0031-z"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2004.05.009"}],"container-title":["Journal of Diabetes Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2020\/2408240.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2020\/2408240.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2020\/2408240.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T23:30:38Z","timestamp":1585956638000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jdr\/2020\/2408240\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,3]]},"references-count":39,"alternative-id":["2408240","2408240"],"URL":"https:\/\/doi.org\/10.1155\/2020\/2408240","relation":{},"ISSN":["2314-6745","2314-6753"],"issn-type":[{"value":"2314-6745","type":"print"},{"value":"2314-6753","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,3]]}}}