{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:51:11Z","timestamp":1772164271554,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T00:00:00Z","timestamp":1600300800000},"content-version":"vor","delay-in-days":260,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    The novel coronavirus disease (COVID\u201019) caused by severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) has previously never been identified with humans, thereby creating devastation in public health. The need for an effective vaccine to curb this pandemic cannot be overemphasized. In view of this, we designed a subcomponent antigenic peptide vaccine targeting the N\u2010terminal (NT) and C\u2010terminal (CT) RNA binding domains of the nucleocapsid protein that aid in viral replication. Promising antigenic B cell and T cell epitopes were predicted using computational pipelines. The peptides \u201cRIRGGDGKMKDL\u201d and \u201cAFGRRGPEQTQGNFG\u201d were the B cell linear epitopes with good antigenic index and nonallergenic property. Two CD8\n                    <jats:sup>+<\/jats:sup>\n                    and Three CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell epitopes were also selected considering their safe immunogenic profiling such as allergenicity, antigen level conservancy, antigenicity, peptide toxicity, and putative restrictions to a number of MHC\u2010I and MHC\u2010II alleles. With these selected epitopes, a nonallergenic chimeric peptide vaccine incapable of inducing a type II hypersensitivity reaction was constructed. The molecular interaction between the Toll\u2010like receptor\u20105 (TLR5) which was triggered by the vaccine was analyzed by molecular docking and scrutinized using dynamics simulation. Finally,\n                    <jats:italic>in silico<\/jats:italic>\n                    cloning was performed to ensure the expression and translation efficiency of the vaccine, utilizing the pET\u201028a vector. This research, therefore, provides a guide for experimental investigation and validation.\n                  <\/jats:p>","DOI":"10.1155\/2020\/2837670","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T19:30:50Z","timestamp":1600371050000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Attenuated Subcomponent Vaccine Design Targeting the SARS\u2010CoV\u20102 Nucleocapsid Phosphoprotein RNA Binding Domain:\n                    <i>In Silico<\/i>\n                    Analysis"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-6798","authenticated-orcid":false,"given":"Onyeka S.","family":"Chukwudozie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca C.","family":"Chukwuanukwu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Onyekachi O.","family":"Iroanya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel M.","family":"Eze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent C.","family":"Duru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Temiloluwa O.","family":"Dele-Alimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Busuyi D.","family":"Kehinde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiwo T.","family":"Bankole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perpetua C.","family":"Obi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth U.","family":"Okinedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,17]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.23750\/abm.v91i1.9397"},{"key":"e_1_2_8_2_2","volume-title":"Coronavirus disease 2019 (COVID-19): Situation report \u2013 88","author":"World Health Organisation","year":"2020"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aivir.2016.08.008"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.12.009"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.08.032"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30251-8"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.01.058"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11373-005-9035-9"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065045"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00473-12"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M708033200"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602107200"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12030254"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-890-0:571"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030091"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.2174\/1875035400801010022"},{"key":"e_1_2_8_17_2","first-page":"82","article-title":"Pymol: an open-source molecular graphics tool","volume":"40","author":"DeLano W. 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