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In this work, effects of a<jats:italic>Clinopodium tomentosum<\/jats:italic>ethanolic extract (CTEE), prepared from aerial parts of the plant, were investigated on vascular endothelium functions. In particularly, angiogenesis activity was evaluated, using primary cultures of porcine aortic endothelial cells (pAECs). Cells were cultured for 24\u2009h in the presence of CTEE different concentrations (10, 25, 50, and 100\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml); no viability alterations were found in the 10-50\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml range, while a slight, but significant, proliferative effect was observed at the highest dose. In addition, treatment with CTEE was able to rescue LPS-induced injury in terms of cell viability. The CTEE ability to affect angiogenesis was evaluated by scratch test analysis and by an<jats:italic>in vitro<\/jats:italic>capillary-like network assay. Treatment with 25-50\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml of extract caused a significant increase in pAEC\u2019s migration and tube formation capabilities compared to untreated cells, as results from the increased master junctions\u2019 number. On the other hand, CTEE at 100\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml did not induce the same effects. Quantitative PCR data demonstrated that FLK-1 mRNA expression significantly increased at a CTEE dose of 25\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml. The CTEE phytochemical composition was assessed through HPLC-DAD; rosmarinic acid among phenolic acids and hesperidin among flavonoids were found as major phenolic components. Total phenolic content and total flavonoid content assays showed that flavonoids are the most abundant class of polyphenols. The CTEE antioxidant activity was also showed by means of the DPPH and ORAC assays. Results indicate that CTEE possesses an angiogenic capacity in a dose-dependent manner; this represents an initial step in elucidating the mechanism of the therapeutic use of the plant.<\/jats:p>","DOI":"10.1155\/2020\/2984613","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T23:49:51Z","timestamp":1597794591000},"page":"1-11","source":"Crossref","is-referenced-by-count":9,"title":["<i>Clinopodium tomentosum<\/i>(Kunth) Govaerts Leaf Extract Influences<i>in vitro<\/i>Cell Proliferation and Angiogenesis on Primary Cultures of Porcine Aortic Endothelial Cells"],"prefix":"10.1155","volume":"2020","author":[{"given":"Irvin","family":"Tubon","sequence":"first","affiliation":[{"name":"Grupo de investigaci\u00f3n GITAFEC, Escuela de Bioqu\u00edmica y Farmacia, Facultad de Ciencias, Escuela Superior Polit\u00e9cnica de Chimborazo, Riobamba. 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