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Curcumin is notable for its biological activities against various viral diseases; however, its poor bioavailability has hindered its potential in therapeutic application. In this study, curcumin was encapsulated in chitosan nanoparticles to improve its bioavailability. Curcumin\u2010encapsulated chitosan (Cur\u2010CS) nanoparticles were synthesised based on the ionic gelation technique and were spherical and cuboidal in shape, with an average particle size of 330\u2009nm and +42\u2009mV in zeta potential. The nanoparticles exerted lower toxicity in Crandell\u2010Rees feline kidney (CrFK) cells and enhanced antiviral activities with a selective index (SI) value three times higher than that of curcumin. Feline\u2010specific bead\u2010based multiplex immunoassay and qPCR were used to examine their modulatory effects on proinflammatory cytokines, including tumour necrosis factor (TNF)<jats:italic>\u03b1<\/jats:italic>, interleukin\u2010 (IL\u2010) 6, and IL\u20101<jats:italic>\u03b2<\/jats:italic>. There were significant decrements in IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20106, and TNF<jats:italic>\u03b1<\/jats:italic> expression in both curcumin and Cur\u2010CS nanoparticles. Based on the multiplex immunoassay, curcumin and the Cur\u2010CS nanoparticles could lower the immune\u2010related proteins in FIP virus (FIPV) infection. The single\u2010 and multiple\u2010dose pharmacokinetics profiles of curcumin and the Cur\u2010CS nanoparticles were determined by high\u2010performance liquid chromatography (HPLC). Oral delivery of the Cur\u2010CS nanoparticles to cats showed enhanced bioavailability with a maximum plasma concentration (<jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub>) value of 621.5\u2009ng\/mL. Incorporating chitosan nanoparticles to deliver curcumin improved the oral bioavailability and antiviral effects of curcumin against FIPV infection. This study provides evidence for the potential of Cur\u2010CS nanoparticles as a supplementary treatment of FIP.<\/jats:p>","DOI":"10.1155\/2020\/3012198","type":"journal-article","created":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T23:30:39Z","timestamp":1590967839000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>In Vitro<\/i> Evaluation of Curcumin\u2010Encapsulated Chitosan Nanoparticles against Feline Infectious Peritonitis Virus and Pharmacokinetics Study in Cats"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3827-3069","authenticated-orcid":false,"given":"Shing Wei","family":"Ng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7240-9882","authenticated-orcid":false,"given":"Gayathri 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