{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:14:32Z","timestamp":1753884872030,"version":"3.41.2"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"vor","delay-in-days":99,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["17140902700","15411950507"],"award-info":[{"award-number":["17140902700","15411950507"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. This study aimed at presenting a novel method of developing a porcine model of portal vein thrombosis (PVT) in cirrhosis by intravenous administration of thrombin and insertion of a fibered coil. We further investigated changes of biochemical parameters, coagulation, and proinflammatory cytokine expression in the cirrhosis\u2010PVT group. <jats:italic>Methods<\/jats:italic>. Twelve male pigs were randomized into the control group (<jats:italic>n<\/jats:italic> = 3) and cirrhosis group (<jats:italic>n<\/jats:italic> = 9). In cirrhotic pigs, three were randomly selected to establish PVT by ultrasound\u2010guided percutaneous puncture of the main portal vein (MPV) followed by intravenous thrombin administration and fibered coil insertion. Thrombosis in the MPV was detected by abdominal enhanced computer tomography (CT). The changes of hepatic function, coagulation system, and inflammation cytokines were compared among normal, cirrhosis, and cirrhosis with PVT groups. <jats:italic>Results<\/jats:italic>. As manifested by the presence of a filling defect in MPV on portal venous\u2010phase CT angiography, fibrin thrombi were formed in the MPV in cirrhotic pigs within one week and persisted for four weeks. Five weeks after surgery, abnormal liver functions occurred in association with PVT formation in cirrhosis. Both coagulation and thromboelastography parameters showed that cirrhosis\u2010PVT pigs exhibited a procoagulant state through hyperfunction of platelets and clotting factors. Interleukin 6 (IL\u20106) as a potential inflammatory marker stimulated PVT\u2010mediated inflammation activation in cirrhosis. <jats:italic>Conclusions<\/jats:italic>. Our study provides <jats:italic>in vivo<\/jats:italic> evidence that intravenous injection of a coil and thrombin into MPV under interventional guided devices enables a feasible method in thrombus creation. Further exploration and validation of large\u2010sample cases are required to characterize utilities of this model.<\/jats:p>","DOI":"10.1155\/2020\/3086906","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T23:30:57Z","timestamp":1586475057000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Preclinical Porcine Model of Portal Vein Thrombosis in Liver Cirrhosis"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4628-5729","authenticated-orcid":false,"given":"Rui","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenxin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-yi","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-qin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2301-147X","authenticated-orcid":false,"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-ying","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7413-2685","authenticated-orcid":false,"given":"Jian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-9198","authenticated-orcid":false,"given":"Shi-yao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,9]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2011.12.034"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.01.001"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/13651820701599033"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000000785"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2006.02820.x"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2010.06.029"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v24.i39.4419"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1011170"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.05.034"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2010.00628.x"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.5604\/01.3001.0010.0296"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6346(03)00208-0"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162144"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2006.01308.x"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.220.1.r01jl28135"},{"key":"e_1_2_11_16_2","first-page":"231","article-title":"Preparation of porcine model of hepatic cirrhosis by peritoneal injection of CCL4 assisted with simple diet control","volume":"15","author":"Zhang J. 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