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As the most common biliary tract tumor, gallbladder cancer (GBC) seriously harms human health while the function of ARAF in GBC remains elusive. Here, we found that ARAF expression was upregulated in gallbladder cancer tissues. <jats:italic>In vitro<\/jats:italic>, ARAF silencing mediated by RNA interference effectively inhibited cell proliferation, colony formation, migration, and invasion of GBC cells. Moreover, knocking down ARAF suppressed tumor growth <jats:italic>in vivo<\/jats:italic>. Our results indicated that ARAF functions as an oncogene in GBC and, thus, could be a potential therapeutic target for GBC.<\/jats:p>","DOI":"10.1155\/2020\/3235786","type":"journal-article","created":{"date-parts":[[2020,8,19]],"date-time":"2020-08-19T23:32:36Z","timestamp":1597879956000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Silencing ARAF Suppresses the Malignant Phenotypes of Gallbladder Cancer Cells"],"prefix":"10.1155","volume":"2020","author":[{"given":"Weiguo","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenhao","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiguang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenda","family":"Cen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiandong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6661-6647","authenticated-orcid":false,"given":"Jianhua","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8350-0227","authenticated-orcid":false,"given":"Baochun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,19]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2011.07048.x"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.2147\/CLEP.S37357"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-016-0370-7"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i22.3978"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2304-3881.2014.09.03"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1586\/17474124.2016.1109445"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(97)80061-0"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2004.09.022"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.330"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-018-2616-4"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2018.08.013"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09004"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2005484"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI72763"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_16_2","first-page":"4109","article-title":"Growth and metastasis of tumor cells isolated from a human renal cell carcinoma implanted into different organs of nude mice","volume":"46","author":"Naito S.","year":"1986","journal-title":"Cancer Research"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13499"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2015.1102858"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208707"},{"key":"e_1_2_9_20_2","first-page":"383","article-title":"Selective inhibition of A-Raf and C-Raf mRNA expression by antisense oligodeoxynucleotides in rat vascular smooth muscle cells: role of A-Raf and C-Raf in serum-induced proliferation","volume":"51","author":"Cioffi C. 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