{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:46:11Z","timestamp":1765295171766,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T00:00:00Z","timestamp":1599436800000},"content-version":"vor","delay-in-days":250,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860512"],"award-info":[{"award-number":["81860512"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Hepatocellular carcinoma (HCC) is one of the most highly aggressive cancer worldwide with an extremely poor prognosis. Evidence has revealed that <jats:italic>microRNA-587<\/jats:italic> (<jats:italic>miR-587<\/jats:italic>) is abnormally expressed in a series of cancers. However, its expressions and functions in HCC have not been clearly acknowledged. <jats:italic>Methods<\/jats:italic>. We detected the expression level of <jats:italic>miR-587<\/jats:italic> both in the Gene Expression Omnibus (GEO) database and 86 paired clinical HCC tissues together with paired adjacent normal tissues by quantitative real\u2010time PCR (qRT\u2010PCR). Afterwards, the transfected HCC cell line SMMC\u20107721 cells were collected for the cell proliferation assay, cell\u2010cycle arrest, cell migration, and invasion assays to explore the roles of <jats:italic>miR-587<\/jats:italic> in regulating cellular function. In addition, bioinformatics analysis, combined with qRT\u2010PCR and dual\u2010luciferase reporter assays, were performed to confirm whether ribosomal protein SA (<jats:italic>RPSA<\/jats:italic>) mRNA was the direct target gene of <jats:italic>miR-587<\/jats:italic>. Moreover, the Cancer Genome Atlas (TCGA) and GEO databases as well as 86 paired clinical HCC tissues were used to verify the negative regulation between <jats:italic>miR-587<\/jats:italic> and <jats:italic>RPSA<\/jats:italic>. <jats:italic>Results<\/jats:italic>. In the present study, both the GEO database (GSE36915 and GSE74618) analysis and qRT\u2010PCR analysis of 86 paired clinical tissues showed that <jats:italic>miR-587<\/jats:italic> was significantly downregulated in HCC tissues. The overexpression of <jats:italic>miR-587<\/jats:italic> inhibited proliferation, cell cycle, migration, and invasion in SMMC\u20107721 cells. In addition, miR\u2010587 directly interacted with the 3\u2009<jats:sup>\u2032<\/jats:sup>\u2010untranslated region (UTR) of <jats:italic>RPSA<\/jats:italic>. Moreover, <jats:italic>miR-587<\/jats:italic> overexpression directly suppressed <jats:italic>RPSA<\/jats:italic> expression, and the two genes were inversely expressed in HCC based on the analyses in TCGA and GEO (GSE36376) databases and qPCR analysis of 86 paired clinical tissues. <jats:italic>Conclusion<\/jats:italic>. Our results demonstrate that <jats:italic>miR-587<\/jats:italic> is downexpressed in HCC and regulates the cellular function by targeting <jats:italic>RPSA<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2020\/3280530","type":"journal-article","created":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T23:31:09Z","timestamp":1599521469000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>MicroRNA-587<\/i> Functions as a Tumor Suppressor in Hepatocellular Carcinoma by Targeting Ribosomal Protein 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