{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T14:21:31Z","timestamp":1772720491680,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"vor","delay-in-days":301,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Adrenocortical carcinoma (ACC) is a rare but clinically aggressive endocrine malignancy. Circular RNAs (circRNAs) were found to play key roles in tumorigenesis. In the current study, we aimed to investigate the functions and mechanisms of a novel circRNA, circ\u2010CCAC1, in ACC cells. circ\u2010CCAC1 expression levels in ACC tissue specimens and cell lines were evaluated by RT\u2010qPCR. Kaplan\u2010Meier analysis was applied to explore the relationship between circ\u2010CCAC1 and patients\u2019 prognosis. Cell counting kit\u20108 (CCK\u20108), colony formation, acridine orange\/ethidium bromide (AO\/EB) double fluorescence staining, and Transwell assays were performed to evaluate the functions of circ\u2010CCAC1 in ACC cells. Bioinformatics analysis and a dual\u2010luciferase reporter assay were utilized to explore the mechanisms of circ\u2010CCAC1. As a result, circ\u2010CCAC1 was overexpressed in ACC tissue samples and cell lines and correlated with poor prognosis. Gain\u2010 and loss\u2010of\u2010function tests demonstrated that circ\u2010CCAC1 acted as an oncogene in ACC. What is more, circ\u2010CCAC1 enhanced C22orf46 expression by sponging miR\u2010514a\u20105p in ACC cells. A rescue assay illustrated that circ\u2010CCAC1 facilitated ACC progression through miR\u2010514a\u20105p\/C22orf46 signaling. To sum up, we identified a novel circRNA, circ\u2010CCAC1, which may be used as a potential therapeutic target for ACC.<\/jats:p>","DOI":"10.1155\/2020\/3501451","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T00:05:14Z","timestamp":1603929914000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Circular RNA circ\u2010CCAC1 Facilitates Adrenocortical Carcinoma Cell Proliferation, Migration, and Invasion through Regulating the miR\u2010514a\u20105p\/C22orf46 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