{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:56:41Z","timestamp":1779148601105,"version":"3.51.4"},"reference-count":62,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Western Norway Regional Health Authority","award":["911802"],"award-info":[{"award-number":["911802"]}]},{"DOI":"10.13039\/501100005036","name":"Universitetet i Bergen","doi-asserted-by":"publisher","award":["911802"],"award-info":[{"award-number":["911802"]}],"id":[{"id":"10.13039\/501100005036","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gastroenterology Research and Practice"],"published-print":{"date-parts":[[2020,2,4]]},"abstract":"Altered densities of enteroendocrine cells play an important role in patients with irritable bowel syndrome (IBS). Uroguanylin activates guanylate cyclase-C to regulate intestinal electrolyte and water transport. Aim<\/jats:italic>. To quantify uroguanylin immunoreactive cells density in the duodenum of diarrhea-predominant IBS (IBS-D) patients compared to controls and to investigate the effect of fecal microbiota transplantation (FMT) on these cell densities. Method<\/jats:italic>. Twelve patients with IBS-D according to Rome III criteria were included. The cause was identified as post infectious (PI, n<\/mml:mi>=<\/mml:mo>6<\/mml:mn><\/mml:math>) or idiopathic (n<\/mml:mi>=<\/mml:mo>6<\/mml:mn><\/mml:math>). They completed the IBS-symptom questionnaire before and 3 weeks after FMT. Thirty grams of fresh feces donated from healthy relatives were diluted with 60\u2009ml normal saline and instilled via endoscope into the duodenum. Biopsies were taken from the patients\u2019 duodenum before and 3 weeks after FMT. Duodenal biopsies taken from eight healthy controls were also included. The biopsies were immunostained for uroguanylin and quantified using computerized image analysis. Results<\/jats:italic>. Uroguanylin immunoreactive cells were found both in duodenal villi and crypts in both controls and IBS-D patients. The densities of uroguanylin immunoreactive cells were significantly lower in the villi (P<\/mml:mi><<\/mml:mo>0.0001<\/mml:mn><\/mml:math>) and higher in the crypts (P<\/mml:mi><<\/mml:mo>0.0001<\/mml:mn><\/mml:math>) for the patients than the controls. Following FMT, the densities of uroguanylin immunoreactive cells for the total group and idiopathic subgroup decreased significantly in the duodenal crypts (P<\/mml:mi>=<\/mml:mo>0.049<\/mml:mn><\/mml:math> and 0.04, respectively) but not in the villi. No significant changes were shown in the PI-IBS subgroups. The cells density in only the crypts correlated with diarrhea (r<\/mml:mi>=<\/mml:mo>0.97<\/mml:mn><\/mml:math>, P<\/mml:mi>=<\/mml:mo>0.001<\/mml:mn><\/mml:math>) and bloating (r<\/mml:mi>=<\/mml:mo>\u2013<\/mml:mo>0.91<\/mml:mn><\/mml:math>, P<\/mml:mi>=<\/mml:mo>0.01<\/mml:mn><\/mml:math>) in the PI-IBS subgroup before FMT and with abdominal pain (r<\/mml:mi>=<\/mml:mo>0.63<\/mml:mn><\/mml:math>, P<\/mml:mi>=<\/mml:mo>0.03<\/mml:mn><\/mml:math>) in the total group of IBS-D patients after FMT. Conclusion<\/jats:italic>. Altered uroguanylin immunoreactive cells density was found in IBS-D patients compared to controls. Changes in these cells density following FMT correlated with IBS symptoms (diarrhea, bloating, and abdominal pain).<\/jats:p>","DOI":"10.1155\/2020\/3520686","type":"journal-article","created":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T18:30:32Z","timestamp":1580841032000},"page":"1-9","source":"Crossref","is-referenced-by-count":6,"title":["Abnormal Uroguanylin Immunoreactive Cells Density in the Duodenum of Patients with Diarrhea-Predominant Irritable Bowel Syndrome Changes following Fecal Microbiota Transplantation"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7983-3707","authenticated-orcid":true,"given":"Tarek","family":"Mazzawi","sequence":"first","affiliation":[{"name":"Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway"},{"name":"Department of Clinical Medicine, University of Bergen, Bergen, 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