{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T20:10:57Z","timestamp":1770754257645,"version":"3.50.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T00:00:00Z","timestamp":1593648000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81730028"],"award-info":[{"award-number":["81730028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["14DZ2260300"],"award-info":[{"award-number":["14DZ2260300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases","award":["81730028"],"award-info":[{"award-number":["81730028"]}]},{"name":"Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases","award":["14DZ2260300"],"award-info":[{"award-number":["14DZ2260300"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neural Plasticity"],"published-print":{"date-parts":[[2020,7,2]]},"abstract":"<jats:p>The prestin-based active process in the mammalian outer hair cells (OHCs) is believed to play a crucial role in auditory signal amplification in the cochlea. Prestin belongs to an anion transporter family (SLC26A). It is densely expressed in the OHC lateral plasma membrane and functions as a voltage-dependent motor protein. Analog genes can be found in the genome of nonmammalian species, but their functions in hearing are poorly understood. In the present study, we used the gerbil prestin sequence as a template and identified an analog gene in the bullfrog genome. We expressed the gene in a stable cell line (HEK293T) and performed patch-clamp recording. We found that these cells exhibited prominent nonlinear capacitance (NLC), a widely accepted assay for prestin functioning as a motor protein. Upon close examination, the key parameters of this NLC are comparable to that conferred by the gerbil prestin, and nontransfected cells failed to display NLC. Lastly, we performed patch-clamp recording in HCs of all three hearing organs in bullfrog. HCs in both the sacculus and the amphibian papilla exhibited a capacitance profile that is similar to NLC while HCs in the basilar papilla showed no sign of NLC. Whether or not this NLC-like capacitance change is involved in auditory signal amplification certainly requires further examination; our results represent the first and necessary step in revealing possible roles of prestin in the active hearing processes found in many nonmammalian species.<\/jats:p>","DOI":"10.1155\/2020\/3570732","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T23:32:46Z","timestamp":1593732766000},"page":"1-9","source":"Crossref","is-referenced-by-count":1,"title":["An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs"],"prefix":"10.1155","volume":"2020","author":[{"given":"Zhongying","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"},{"name":"Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China"},{"name":"Shanghai Key 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Shanghai, China"},{"name":"Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8802-8566","authenticated-orcid":true,"given":"Zhiwu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People\u2019s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"},{"name":"Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China"},{"name":"Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, 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