{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T21:05:22Z","timestamp":1768251922680,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"vor","delay-in-days":90,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703990"],"award-info":[{"award-number":["81703990"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>The present study investigated the effect of Chinese medicine Sini\u2010San (SNS) on visceral hypersensitivity in a rat model of functional dyspepsia (FD), and it explored related underlying mechanisms. The rat model of FD was developed by combining neonatal iodoacetamide (IA) treatment and adult tail\u2010clamping. After SNS treatment, the behavior and electromyographic testing were performed to evaluate the visceromotor responses of rats to gastric distention. Immunofluorescence was used to detect the distribution of iNOS\u2010positive cells in the spinal dorsal horn, while the real\u2010time quantitative PCR and western blot were used for detection of the gene expression of c\u2010fos, iNOS, and GABAb and protein levels of iNOS and GABAb in the spinal dorsal horn, respectively. The protein concentration of cGMP and PKG proteins in the spinal dorsal horn were quantified by enzyme\u2010linked immunosorbent assay. In this study, SNS treatment significantly reduced the behavioral score and electromyographic response to graded intragastric distension pressure. The middle\u2010dose of SNS treatment significantly reduced the distribution of iNOS\u2010positive cells in the spinal dorsal horn of FD model rats. The gene expression of c\u2010fos, iNOS, and GABAb and the protein contents of iNOS, GABAb, cGMP, and PKG in the spinal dorsal horn of FD model rats were restored to a normal level by middle\u2010dose of SNS treatment. Our results suggest that Sini\u2010San may alleviate the visceral hypersensitivity in FD model rats via regulation of the NO\/cGMP\/PKG pathway in the spinal dorsal horn.<\/jats:p>","DOI":"10.1155\/2020\/3575231","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T23:45:48Z","timestamp":1585698348000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Sini\u2010San Regulates the NO\u2010cGMP\u2010PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8911-7031","authenticated-orcid":false,"given":"Zhenyu","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1837-1091","authenticated-orcid":false,"given":"Xiaofang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0457-1727","authenticated-orcid":false,"given":"Shengsheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,31]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2016.02.011"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12657"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00840"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/753656"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.12.003"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2009.11.034"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000832"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.5754\/hge11246"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13319"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/2040622317725479"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/812143"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-013-1503-x"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i44.16739"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12348"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nem101"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1177\/1756284818785573"},{"key":"e_1_2_9_17_2","first-page":"833","article-title":"Study on distribution characteristic of syndrome of 565 cases of functional dyspepsia by twice differentiation of symptoms and signs based on the \u201ccold, heat, deficiency, excess\u201d","volume":"23","author":"Zhang S. 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