{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T05:24:47Z","timestamp":1774502687498,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T00:00:00Z","timestamp":1586476800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bombay College of Pharmacy, Mumbai","award":["VI-D&P\/552\/2016\u201317\/TDT(G)"],"award-info":[{"award-number":["VI-D&P\/552\/2016\u201317\/TDT(G)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Analytical Chemistry"],"published-print":{"date-parts":[[2020,4,10]]},"abstract":"<jats:p>Low-dose thiazide and thiazide-like diuretics are widely used as first-line therapy for hypertension. Chlorthalidone, a monosulfamyl diuretic, is frequently prescribed in cases of hypertension and congestive heart failure. In this research paper, an improved reverse-phase HPLC method was developed for the simultaneous identification and quantitation of pharmacopoeia-listed and in-house process- and degradation-related impurities of chlorthalidone in bulk drug and formulations. Chromatographic separation was carried out on a C<jats:sub>8<\/jats:sub> column (250\u2009\u00d7\u20094.6\u2009mm; \u20185\u2009<jats:italic>\u03bc<\/jats:italic>m particle size) at a flow rate of 1.4\u2009mL\/min with a 220\u2009nm detection wavelength. Mobile phase A consisted of buffer solution (diammonium hydrogen orthophosphate (10\u2009mM, pH 5.5)) and methanol at a 65\u2009:\u200935 ratio (v\/v), and mobile phase B consisted of buffer solution and methanol at a 50\u2009:\u200950 ratio (v\/v). The API and formulation were subjected to stress conditions such as acid, alkali, oxidation, thermal, and photolytic conditions. Validation studies for the in-house process impurities were performed for specificity, limit of detection (LOD), limit of quantitation (LOQ), linearity, precision, accuracy, and robustness. Thus, an improved RP-HPLC method capable of good separation of all known and unknown impurities with acceptable resolution and tailing factor was developed.<\/jats:p>","DOI":"10.1155\/2020\/3593805","type":"journal-article","created":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T23:33:22Z","timestamp":1586561602000},"page":"1-11","source":"Crossref","is-referenced-by-count":13,"title":["A Validated RP-HPLC Stability Method for the Estimation of Chlorthalidone and Its Process-Related Impurities in an API and Tablet Formulation"],"prefix":"10.1155","volume":"2020","author":[{"given":"Chaitali","family":"Kharat","sequence":"first","affiliation":[{"name":"Bombay College of Pharmacy, Kalina, Santacruz East, Mumbai 4000098, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8838-7198","authenticated-orcid":true,"given":"Vaishali A.","family":"Shirsat","sequence":"additional","affiliation":[{"name":"Bombay College of Pharmacy, Kalina, Santacruz East, Mumbai 4000098, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yogita M.","family":"Kodgule","sequence":"additional","affiliation":[{"name":"IQGEN-X Pharma Pvt. Ltd., A-165, Khairane Road, Sector 2, Kopar Khairane, Navi Mumbai 400710, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandar","family":"Kodgule","sequence":"additional","affiliation":[{"name":"IQGEN-X Pharma Pvt. Ltd., A-165, Khairane Road, Sector 2, Kopar Khairane, Navi Mumbai 400710, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"2","volume":"4","year":"2006"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/9781118971147.ch5"},{"key":"4","year":"2016"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/s0039-9140(96)02179-0"},{"key":"6","year":"2006"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.4103\/0110-5558.72422"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1080\/10826070701191151"},{"issue":"7","key":"9","first-page":"254","volume":"6","year":"2014","journal-title":"Journal of Pharmaceutical Sciences and Research"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(03)00293-0"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1080\/01483919008049020"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2013.09.003"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.07.013"},{"key":"14","year":"2007"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1586\/erc.09.31"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1177\/107424840501000406"},{"key":"17","first-page":"1","volume-title":"Chlorthalidone","volume":"14","year":"1985"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-014-8085-0"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-008-0755-7"},{"key":"20","first-page":"226","volume":"26","year":"2014","journal-title":"International Journal of Pharmaceutical Sciences Review and Research"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4286482"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(86)80045-0"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1134\/s1061934818060138"},{"key":"24","volume":"2","year":"2010","journal-title":"Council of Europe: European Directoratefor the Quality of Medicines and HealthCare"}],"container-title":["International Journal of Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijac\/2020\/3593805.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijac\/2020\/3593805.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijac\/2020\/3593805.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T23:33:26Z","timestamp":1586561606000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ijac\/2020\/3593805\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,10]]},"references-count":23,"alternative-id":["3593805","3593805"],"URL":"https:\/\/doi.org\/10.1155\/2020\/3593805","relation":{},"ISSN":["1687-8760","1687-8779"],"issn-type":[{"value":"1687-8760","type":"print"},{"value":"1687-8779","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,10]]}}}