{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:15:26Z","timestamp":1766870126393,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fujian Province Science and Technology Planning Foundations","award":["2015J01514","2017Z36"],"award-info":[{"award-number":["2015J01514","2017Z36"]}]},{"name":"Quanzhou High Level Talent Innovation and Entrepreneurship Project","award":["2015J01514","2017Z36"],"award-info":[{"award-number":["2015J01514","2017Z36"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2020,4,9]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. This study aimed to detect 5 kinds of genes related to plasmid-mediated quinolone resistance in four species of nonfermenting bacteria with 2 drug resistance phenotypes (multidrug resistance and pandrug resistance), which were <jats:italic>Acinetobacter baumannii<\/jats:italic> (Ab), <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (Pa), <jats:italic>Stenotrophomonas maltophilia<\/jats:italic> (Sm), and <jats:italic>Elizabethkingia meningoseptica<\/jats:italic> (Em). <jats:italic>Methods<\/jats:italic>. The Phoenix NMIC\/ID-109 panel and API 20NE panel were applied to 19 isolated strains, including 6 Ab strains (2 strains with multidrug resistance and 4 strains with pandrug resistance), 6 Pa strains (3 strains with multidrug resistance and 3 strains with pandrug resistance), 4 Sm strains (2 strains with multidrug resistance and 2 strains with pandrug resistance), and 3 Cm strains (2 strains with multidrug resistance and 1 strain with pandrug resistance). After strain identification and drug susceptibility test, PCR was applied to detect 5 genes related to plasmid-mediated quinolone resistance. The genes detected were quinolone resistance A (<jats:italic>qnrA<\/jats:italic>), aminoglycoside acetyltransferase ciprofloxacin resistance variant, <jats:italic>acc(6\u2032)-Ib-cr<\/jats:italic>, and 3 integrons (<jats:italic>intI<\/jats:italic>1, <jats:italic>intI<\/jats:italic>2, and <jats:italic>intI<\/jats:italic>3). The amplified products were analyzed by 1% agarose gel electrophoresis and sequenced. Sequence alignment was carried out using the bioinformatics technique. <jats:italic>Results<\/jats:italic>. Of 19 strains tested, 8 strains carried <jats:italic>acc(6\u2032)-Ib-cr<\/jats:italic> and 6 of them were of pandrug resistance phenotype (3 Ab strains, 2 Pa strains, and 1 Sm strain). The carrying rate of <jats:italic>acc(6\u2032)-Ib-cr<\/jats:italic> was 60.0% for strains of pandrug resistance (6\/10). Two strains were of multidrug resistance (1 Ab strain and 1 Pa strain), and the carrying rate of <jats:italic>acc(6\u2032)-Ib-cr<\/jats:italic> was 22.0% (2\/9). The carrying rate was significantly different between strains of multidrug resistance and pandrug resistance (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>). The class 1 integron was detected in 11 strains, among which 6 strains were of pandrug resistance (3 Ab strains, 2 Pa strains, and 1 Sm strain). The carrying rate of the class 1 integron was 60.0% (6\/10). Five strains were of multidrug resistance (3 Pa strains, 1 Ab strain, and 1 Em strain), and the carrying rate was 55.6% (5\/9). The carrying rate of the class 1 integron was not significantly different between strains of multidrug resistance and pandrug resistance (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&gt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>). Both <jats:italic>acc(6\u2032)-Ib-cr<\/jats:italic> and <jats:italic>intI<\/jats:italic>1 were detected in 6 strains, which were negative for <jats:italic>qnrA<\/jats:italic>, <jats:italic>intI<\/jats:italic>2, and <jats:italic>intI<\/jats:italic>3. <jats:italic>Conclusion<\/jats:italic>. Quinolone resistance of isolated strains was related to <jats:italic>acc(6\u2032)-Ib-cr<\/jats:italic> and <jats:italic>intI<\/jats:italic>1 but not to <jats:italic>qnrA<\/jats:italic>, <jats:italic>intI<\/jats:italic>2, or <jats:italic>intI<\/jats:italic>3. The carrying rate of <jats:italic>acc(6\u2032)-Ib-cr<\/jats:italic> among the strains of pandrug resistance was much higher than that among the strains of multidrug resistance. But, the strains of two drug resistant phenotypes were not significantly different in the carrying rate of <jats:italic>intI<\/jats:italic>1. The detection rates of the two genes were high and similar in Ab and Pa strains. 1 Em strain carried the class 1 integron.<\/jats:p>","DOI":"10.1155\/2020\/3948719","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T23:31:07Z","timestamp":1586475067000},"page":"1-5","source":"Crossref","is-referenced-by-count":0,"title":["Detection of 5 Kinds of Genes Related to Plasmid-Mediated Quinolone Resistance in Four Species of Nonfermenting Bacteria with 2 Drug Resistant Phenotypes"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4146-391X","authenticated-orcid":true,"given":"De-Song","family":"Ming","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Qing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.073262-0"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.5847\/wjem.j.issn.1920-8642.2013.03.002"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2013.09.005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1223-0"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2014.914172"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00024"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.02.011"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.2174\/092986709787581879"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-010-0120-2"},{"year":"2016","key":"10"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00830-08"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2008.00063.x"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.10.002"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.5578\/mb.24126"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2015.09.015"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph120810093"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.18683\/germs.2017.1117"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.5578\/mb.8459"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2016.03.006"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix1052"}],"container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjidmm\/2020\/3948719.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjidmm\/2020\/3948719.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjidmm\/2020\/3948719.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T23:31:10Z","timestamp":1586475070000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/cjidmm\/2020\/3948719\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,9]]},"references-count":20,"alternative-id":["3948719","3948719"],"URL":"https:\/\/doi.org\/10.1155\/2020\/3948719","relation":{},"ISSN":["1712-9532","1918-1493"],"issn-type":[{"type":"print","value":"1712-9532"},{"type":"electronic","value":"1918-1493"}],"subject":[],"published":{"date-parts":[[2020,4,9]]}}}