{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T01:35:23Z","timestamp":1778981723478,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T00:00:00Z","timestamp":1593216000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["JU 426\/5-1"],"award-info":[{"award-number":["JU 426\/5-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CI 203\/2-1"],"award-info":[{"award-number":["CI 203\/2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["JU 426\/5-1"],"award-info":[{"award-number":["JU 426\/5-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CI 203\/2-1"],"award-info":[{"award-number":["CI 203\/2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Humboldt Research Fellowship","award":["JU 426\/5-1"],"award-info":[{"award-number":["JU 426\/5-1"]}]},{"name":"Humboldt Research Fellowship","award":["CI 203\/2-1"],"award-info":[{"award-number":["CI 203\/2-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Oncology"],"published-print":{"date-parts":[[2020,6,27]]},"abstract":"<jats:p>Multiple myeloma (MM) bone disease is characterized by osteolytic bone tissue destruction resulting in bone pain, fractures, vertebral collapse, and spinal cord compression in patients. Upon initial diagnosis of MM, almost 80% of patients suffer from bone disease. Earlier diagnosis and intervention in MM bone disease would potentially improve treatment outcome and patient survival. New preclinical models are needed for developing novel diagnostic markers of bone structural changes as early as possible in the disease course. Here, we report a proof-of-concept, syngeneic, intrafemoral MOPC315.BM MM murine model in skeletally mature BALB\/c mice for detection and characterization of very early changes in the extracellular matrix (ECM) of MM-injected animals. Bioluminescence imaging (BLI) in vivo confirmed myeloma engraftment in 100% of the animals with high osteoclast activity within 21 days after tumor cell inoculation. Early signs of aggressive bone turnover were observed on the outer bone surfaces by high-resolution microcomputed tomography (microCT). Synchrotron phase contrast-enhanced microcomputer tomography (PCE-CT) revealed very local microarchitecture differences highlighting numerous active sites of erosion and new bone at the micrometer scale. Correlative backscattered electron imaging (BSE) and confocal laser scanning microscopy allowed direct comparison of mineralized and nonmineralized matrix changes in the cortical bone. The osteocyte lacunar-canalicular network (OLCN) architecture was disorganized, and irregular-shaped osteocyte lacunae were observed in MM-injected bones after 21 days. Our model provides a potential platform to further evaluate pathological MM bone lesion development at the micro- and ultrastructural levels. These promising results make it possible to combine material science and pharmacological investigations that may improve early detection and treatment of MM bone disease.<\/jats:p>","DOI":"10.1155\/2020\/3985315","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T23:31:37Z","timestamp":1593300697000},"page":"1-12","source":"Crossref","is-referenced-by-count":7,"title":["An Early Myeloma Bone Disease Model in Skeletally Mature Mice as a Platform for Biomaterial Characterization of the Extracellular Matrix"],"prefix":"10.1155","volume":"2020","author":[{"given":"Fani","family":"Ziouti","sequence":"first","affiliation":[{"name":"Department of Internal Medicine II, University Hospital W\u00fcrzburg, Oberd\u00fcrrbacher Stra\u00dfe 6, 97080 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Prates","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, University Hospital W\u00fcrzburg, Oberd\u00fcrrbacher Stra\u00dfe 6, 97080 W\u00fcrzburg, Germany"},{"name":"Department for Operative and Preventive Dentistry, Centrum f\u00fcr Zahn-, Mund- und Kieferheilkunde, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, A\u00dfmannshauser Str. 4-6, 14197 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In\u00e9s","family":"Moreno-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Am M\u00fchlenberg 1, 14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Rack","sequence":"additional","affiliation":[{"name":"ESRF\u2014The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bjarne","family":"Bogen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of Clinical Medicine, University of Oslo and Oslo University Hospital, Sognsvannsveien 20, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amaia","family":"Cipitria","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Am M\u00fchlenberg 1, 14476 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Zaslansky","sequence":"additional","affiliation":[{"name":"Department for Operative and Preventive Dentistry, Centrum f\u00fcr Zahn-, Mund- und Kieferheilkunde, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, A\u00dfmannshauser Str. 4-6, 14197 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8661-7110","authenticated-orcid":true,"given":"Franziska","family":"Jundt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, University Hospital W\u00fcrzburg, 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