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Tuberculosis<\/jats:italic>) is described. In this study, the nanohybrid films NH<jats:sub>2<\/jats:sub>-rGO\/TEMPO-NCC were immobilized onto screen-printed carbon electrode (SPE) via a simple drop-coating method. The electrochemical characterization of the designed electrode was investigated using cyclic voltammetry (CV) and impedance spectroscopy (EIS). Meanwhile, the sensitivity and selectivity of the designed biosensor against<jats:italic>M. tuberculosis<\/jats:italic>were measured by the differential pulse voltammetry (DPV). The response of the PNA probe-modified (NH2-rGO)\/TEMPO-NCC demonstrated that the fabricated biosensor was able to distinguish between complementary, noncomplementary, and one-base mismatch DNA sequences using methylene blue (MB) as the electrochemical indicator. The developed electrochemical biosensor exhibited a linear calibration curve in the concentration range of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn>1<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mo>\u2212<\/mml:mo><mml:mn>8<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mtext>\u2009<\/mml:mtext><mml:mtext>M<\/mml:mtext><\/mml:math>to<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn>1<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mo>\u2212<\/mml:mo><mml:mn>13<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mtext>\u2009<\/mml:mtext><mml:mtext>M<\/mml:mtext><\/mml:math>with the limit of detection of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mn>3.14<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mo>\u2212<\/mml:mo><mml:mn>14<\/mml:mn><\/mml:mrow><\/mml:msup><mml:mtext>\u2009<\/mml:mtext><mml:mtext>M<\/mml:mtext><\/mml:math>. The developed electrochemical biosensor has also been tested with a polymerase chain reaction (PCR) product of<jats:italic>M. tuberculosis<\/jats:italic>DNA which has shown successful results in distinguishing between negative and positive samples of M. tuberculosis.<\/jats:p>","DOI":"10.1155\/2020\/4051474","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T23:31:06Z","timestamp":1579649466000},"page":"1-11","source":"Crossref","is-referenced-by-count":56,"title":["Reduced Graphene Oxide\/TEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination of<i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1155","volume":"2020","author":[{"given":"Mohd Hazani Mat","family":"Zaid","sequence":"first","affiliation":[{"name":"Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdan, Selangor D.E., Malaysia"},{"name":"Centre for Advanced Materials and Renewable Resources, Faculty of Science and Technology, Universiti 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