{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T20:34:48Z","timestamp":1768595688845,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T00:00:00Z","timestamp":1588377600000},"content-version":"vor","delay-in-days":122,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000297","name":"Royal College of Surgeons of England","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000297","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000341","name":"Arthritis Research UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000769","name":"University of Oxford","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Adult articular cartilage synthesises very little type II collagen in comparison to young cartilage. The age\u2010related difference in collagen II synthesis is poorly understood. This is the first systematic investigation of age\u2010related differences in extracellular matrix synthesis in fresh articular cartilage and following isolation of chondrocytes. A histological comparison of 3\u2010year\u2010old skeletally mature and 6\u2010month\u2010old juvenile porcine cartilage was made. Differences in collagen II, aggrecan, and Sox5, 6, and 9 mRNA and protein expression and mRNA stability were measured. Adult cartilage was found to be thinner than juvenile cartilage but with similar chondrocyte density. Procollagen <jats:italic>\u03b1<\/jats:italic>1(II) and Sox9 mRNA levels were 10\u2010fold and 3\u2010fold reduced in adult cartilage. Sox9 protein was halved and collagen II protein synthesis was almost undetectable and calculated to be at least 30\u2010fold reduced. Aggrecan expression did not differ. Isolation of chondrocytes caused a drop in procollagen <jats:italic>\u03b1<\/jats:italic>1(II) and Sox9 mRNA in both adult and juvenile cells along with a marked reduction in Sox9 mRNA stability. Interestingly, juvenile chondrocytes continued to synthesise collagen II protein with mRNA levels similar to those seen in adult articular cartilage. Age\u2010related differences in collagen II protein synthesis are due to both transcriptional and posttranscription regulation. A better understanding of these regulatory mechanisms would be an important step in improving current cartilage regeneration techniques.<\/jats:p>","DOI":"10.1155\/2020\/4060135","type":"journal-article","created":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T23:30:25Z","timestamp":1588462225000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Effects of Age and Cell Isolation on Collagen II Synthesis by Articular Chondrocytes: Evidence for Transcriptional and Posttranscriptional Regulation"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-1343","authenticated-orcid":false,"given":"Vipin","family":"Asopa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3412-5712","authenticated-orcid":false,"given":"Tonia","family":"Vincent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Saklatvala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,2]]},"reference":[{"key":"e_1_2_10_1_2","first-page":"514","article-title":"Of the structure and diseases of articulating cartilages, by William Hunter, surgeon","volume":"42","author":"Hunter W.","year":"1742","journal-title":"Philosophical Transactions"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200110001-00026"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199801000-00003"},{"key":"e_1_2_10_4_2","first-page":"230","article-title":"Fetal chondral homografts in the repair of articular cartilage defects","volume":"54","author":"Specchia N.","year":"1996","journal-title":"Bulletin\/Hospital for Joint Diseases"},{"key":"e_1_2_10_5_2","first-page":"749","article-title":"Age dependent possibilities for healing in cartilage injuries (experimental investigations with animals) (author\u2019s transl)","volume":"114","author":"Dustmann H. O.","year":"1976","journal-title":"Zeitschrift f\u00fcr Orthop\u00e4die und Ihre Grenzgebiete"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199905)42:5<1003::AID-ANR20>3.0.CO;2-K"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407041200"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.02.010"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2013.08.024"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.05.052"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.24.4.341"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2014.09.002"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20369"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.105.4.975"},{"key":"e_1_2_10_16_2","first-page":"15","article-title":"Growth and skeletal development of the pig","volume":"358","author":"Reiland S.","year":"1978","journal-title":"Acta Radiologica Supplementum"},{"key":"e_1_2_10_17_2","volume-title":"Longevity of Mammals in Captivity: From the Living Collections of the World","author":"Weigl R.","year":"2005"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.17.4.2336"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/0363546513502945"},{"key":"e_1_2_10_20_2","first-page":"753","article-title":"The cell density of human articular and costal cartilage","volume":"101","author":"Stockwell R. A.","year":"1967","journal-title":"Journal of Anatomy"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.15.10738"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/916530"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0945-053x(03)00049-0"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206544200"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407510102"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22878"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.04.437"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2016.1200566"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2052"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.543322"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1159\/000496688"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2016.01.013"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEA.2010.0579"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604322200"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/4060135.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/4060135.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/4060135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T07:41:53Z","timestamp":1735630913000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/4060135"}},"subtitle":[],"editor":[{"given":"Rudolf K.","family":"Braun","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/4060135"],"URL":"https:\/\/doi.org\/10.1155\/2020\/4060135","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"4060135"}}