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Among 112 elderly individuals (\u226560 years of age), 14.28% were positive for at least one enteroparasite, with one individual positive for <jats:italic>S. stercoralis<\/jats:italic>. Sera were evaluated for the presence of anti-<jats:italic>Strongyloides<\/jats:italic> sp. antibodies using total or detergent fraction extracts of <jats:italic>Strongyloides venezuelensis<\/jats:italic>, which presented positivity rates of 19.64% and 10.71%, respectively. An anti-HSP60 single-chain variable fragment from <jats:italic>Strongyloides<\/jats:italic> sp. was used to detect parasite antigens, with 5.36% (6 individuals) of ELISA-positive individuals returning a positive result. While the serological test indicates previous or recent infection and may be limited by antigen purification, the anti-HSP60 method reflects the presence of <jats:italic>Strongyloides<\/jats:italic> sp. immune complexes and exhibits greater sensitivity and specificity. Our results demonstrate the variable occurrence of enteroparasites in elderly individuals residing in long-term nursing homes and validate a novel epidemiological tool to describe infection cases by <jats:italic>Strongyloides<\/jats:italic> sp.<\/jats:p>","DOI":"10.1155\/2020\/4086929","type":"journal-article","created":{"date-parts":[[2020,1,11]],"date-time":"2020-01-11T18:31:01Z","timestamp":1578767461000},"page":"1-6","source":"Crossref","is-referenced-by-count":10,"title":["scFv against HSP60 of <i>Strongyloides<\/i> sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1834-1394","authenticated-orcid":true,"given":"Camila Botelho","family":"Miguel","sequence":"first","affiliation":[{"name":"Federal University of Tri\u00e2ngulo Mineiro (UFTM), 38061-500 Uberaba, MG, Brazil"},{"name":"University Center of Mineiros-Unifimes, 75., 830-000 Mineiros, GO, 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