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The objective of the present study was to compare the proosteogenic and proangiogenic effects of sEVs derived from BMMSCs from rats with steroid\u2010induced ONFH (oBMMSCs\u2010sEVs) and sEVs derived from BMMSCs from normal rats (nBMMSCs\u2010sEVs). BMMSCs were isolated from steroid\u2010induced ONFH rats and healthy rats. sEVs were isolated and characterized by Western blotting analysis of exosomal surface biomarkers and by transmission electron microscopy. The impacts of nBMMSCs\u2010sEVs and oBMMSCs\u2010sEVs on the proliferation and osteogenic differentiation of BMMSCs were determined via cell proliferation assay, alizarin red staining, and alkaline phosphatase activity assay. Enzyme\u2010linked immunosorbent assay and tube formation assay were conducted to investigate the effect of nBMMSCs\u2010sEVs and oBMMSCs\u2010sEVs on the angiogenic potential of human umbilical vein endothelial cells (HUVECs). The expression of relevant genes was detected by quantitative real\u2010time polymerase chain reaction analysis, and the expression of <jats:italic>\u03b2<\/jats:italic>\u2010catenin was detected by immunofluorescence. Both nBMMSCs\u2010sEVs and oBMMSCs\u2010sEVs promoted proliferation, osteogenic differentiation, and <jats:italic>\u03b2<\/jats:italic>\u2010catenin expression of BMMSCs and enhanced angiogenesis of HUVECs. However, compared with nBMMSCs\u2010sEVs, oBMMSCs\u2010sEVs exhibited attenuated effects. Our findings indicated that the proosteogenic and proangiogenic effects of sEVs were partially attenuated in steroid\u2010induced ONFH. Therefore, this study might offer guidance for the selection of source cells for sEV therapy in the future.<\/jats:p>","DOI":"10.1155\/2020\/4176926","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T23:31:41Z","timestamp":1588894301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Proosteogenic and Proangiogenic Effects of Small Extracellular Vesicles Derived from Bone Marrow Mesenchymal Stem Cells Are Attenuated in Steroid\u2010Induced Osteonecrosis of the Femoral 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