{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T04:25:18Z","timestamp":1760502318366,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":328,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>The rise of the emergence of microbial resistance of antibiotics, the dangerous side effects of nonsteroidal anti\u2010inflammatory drugs, and noncompetent medications of Alzheimer\u2019s, Parkinson\u2019s, and other neurodegenerative diseases prompt scientists to search for phytochemicals that could be utilized in the remedy of lethal diseases. <jats:italic>Glechoma curviflora<\/jats:italic> (Boiss.) Kuntze (<jats:italic>Nepeta curviflora<\/jats:italic>) is a medicinal herb growing in the eastern parts of the Mediterranean Sea Basin and is widely consumed as a tea. The leaves of this plant have been traditionally used for the treatment of various infectious diseases. The current research was designed to identify the chemical composition of <jats:italic>Glechoma curviflora<\/jats:italic> (Boiss.) essential oil (EO) and to assess its antibacterial, antifungal, and cyclooxygenase inhibitory activities and the biophysical gating effect on AMPA receptors. Twenty phytochemicals were identified from <jats:italic>G. curviflora<\/jats:italic> leaves and flowers EO amounting to almost 100% of the total constituents using GC\u2010MS technique, of which 1,6\u2010dimethylspiro[4.5]decane (27.51%) <jats:bold>1<\/jats:bold>, caryophyllene oxide (20.08%) <jats:bold>2<\/jats:bold>, and <jats:italic>\u03b2<\/jats:italic>\u2010caryophyllene (18.28%) <jats:bold>3<\/jats:bold> were the main constituents. The biophysical properties\u2019 effect from the plant extract on various AMPA\u2010type receptors expressed in Human Embryonic Kidney (HEK293) cells was assessed by exploiting the whole\u2010cell patch\u2010clamp technique. Microdilution assay was adopted for assessing the antimicrobial property against eight virulent microbial strains whilst the cyclooxygenase inhibition effect was accomplished utilizing COX inhibitory screening colorimetric assay <jats:italic>G. curviflora<\/jats:italic> EO displayed potent activity against <jats:italic>P. aeruginosa<\/jats:italic> (MIC\u2009=\u20091.25\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), <jats:italic>S. sonnei<\/jats:italic> (MIC\u2009=\u20093.12\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), and <jats:italic>E. coli<\/jats:italic> (MIC\u2009=\u20091.25\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), compared with ciprofloxacin (positive control) and potent antibacterial activity against <jats:italic>S. aureus<\/jats:italic>, MRSA, <jats:italic>S. sonnei<\/jats:italic>, <jats:italic>E. coli<\/jats:italic>, and <jats:italic>P. aeruginosa<\/jats:italic> compared to Ampicillin (2nd positive control). It also showed anti\u2010<jats:italic>Candida<\/jats:italic> (MIC\u2009=\u20096.25\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) and antimold (MIC\u2009=\u20093.125\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) activities compared with fluconazole (antifungal positive control). Likewise, our results showed an inhibition and biophysical impact of <jats:italic>G<\/jats:italic>. curviflora on all AMPARs subunits.<\/jats:p>","DOI":"10.1155\/2020\/4195272","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T22:22:07Z","timestamp":1606256527000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Glechoma curviflora<\/i> Volatile Oil from Palestine: Chemical Composition and Neuroprotective, Antimicrobial, and Cyclooxygenase Inhibitory Activities"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7014-6190","authenticated-orcid":false,"given":"Nawaf","family":"Al-Maharik","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2291-6821","authenticated-orcid":false,"given":"Nidal","family":"Jaradat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0702-7834","authenticated-orcid":false,"given":"Mohammad","family":"Qneibi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2083-856X","authenticated-orcid":false,"given":"Murad N.","family":"Abualhasan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nour","family":"Emwas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"volume-title":"Phytochemicals as Lead Compounds for New Drug Discovery","year":"2019","author":"Egbuna C.","key":"e_1_2_10_1_2"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-11020-09664-x"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2019.06.005"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23656"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4034689"},{"key":"e_1_2_10_6_2","first-page":"247","article-title":"Anti-inflammatory and side effects of cyclo-oxygenase inhibitors","volume":"59","author":"Suleyman H.","year":"2007","journal-title":"Pharmacological Reports"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.05.013"},{"key":"e_1_2_10_8_2","first-page":"137","article-title":"Chemical composition and biological activity of essential oils from four Nepeta species and hybrids against Aedes aegypti (L.) 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