{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T10:48:02Z","timestamp":1756464482254,"version":"3.37.3"},"reference-count":67,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T00:00:00Z","timestamp":1579910400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2020,1,25]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. The aim of this study was to investigate the anti-inflammatory effects of the crude extract (CE), derived fraction, and isolated compounds from <jats:italic>Calea pinnatifida<\/jats:italic> leaves in a mouse model of pulmonary neutrophilia. <jats:italic>Methods<\/jats:italic>. The CE and derived fractions, hexane, ethyl acetate, and methanol, were obtained from <jats:italic>C<\/jats:italic>. <jats:italic>pinnatifida<\/jats:italic> leaves. The compounds 3,5- and 4,5-di-<jats:italic>O<\/jats:italic>-<jats:italic>E<\/jats:italic>-caffeoylquinic acids were isolated from the EtOAc fraction using chromatography and were identified using infrared spectroscopic data and nuclear magnetic resonance (<jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C NMR). Leukocytes count, protein concentration of the exudate, myeloperoxidase (MPO) and adenosine deaminase (ADA), and nitrate\/nitrite (NO<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:msub><mml:mrow\/><mml:mrow><mml:mi>x<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:math>), tumor necrosis factor-alpha (TNF-<jats:italic>\u03b1<\/jats:italic>), interleukin-1-beta (IL-1<jats:italic>\u03b2<\/jats:italic>), and interleukin-17A (IL-17A) levels were determined in the pleural fluid leakage after 4\u2009h of pleurisy induction. We also analyzed the effects of isolated compounds on the phosphorylation of both p65 and p38 in the lung tissue. <jats:italic>Results<\/jats:italic>. The CE, its fractions, and isolated compounds inhibited leukocyte activation, protein concentration of the exudate, and MPO, ADA, NO<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:msub><mml:mrow\/><mml:mrow><mml:mi>x<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:math>, TNF-<jats:italic>\u03b1<\/jats:italic>, IL-1<jats:italic>\u03b2<\/jats:italic>, and IL-17A levels. 3,5- and 4,5-di-<jats:italic>O<\/jats:italic>-<jats:italic>E<\/jats:italic>-caffeoylquinic acids also inhibited phosphorylation of both p65 and p38 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). <jats:italic>Conclusion<\/jats:italic>. This study demonstrated that <jats:italic>C<\/jats:italic>. <jats:italic>pinnatifida<\/jats:italic> presents important anti-inflammatory properties by inhibiting activated leukocytes and protein concentration of the exudate. These effects were related to the inhibition of proinflammatory mediators. The dicaffeoylquinic acids may be partially responsible for these anti-inflammatory properties through the inhibition of nuclear transcription factor kappa B and mitogen-activated protein kinase pathways.<\/jats:p>","DOI":"10.1155\/2020\/4620251","type":"journal-article","created":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T18:31:41Z","timestamp":1579977101000},"page":"1-12","source":"Crossref","is-referenced-by-count":6,"title":["Systemic Administration of <i>Calea pinnatifida<\/i> Inhibits Inflammation Induced by Carrageenan in a Murine Model of Pulmonary Neutrophilia"],"prefix":"10.1155","volume":"2020","author":[{"given":"Bruno Matheus","family":"de Campos Facchin","sequence":"first","affiliation":[{"name":"Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia Salvan","family":"da Rosa","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Beatriz Gobbo","family":"Luz","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeo Jim Kinoshita","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamires Cardoso","family":"de Lima","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosana","family":"Casoti","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of S\u00e3o Paulo, Ribeir\u00e3o Preto, SP-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maique Weber","family":"Biavatti","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5220-5396","authenticated-orcid":true,"given":"Eduardo Monguilhott","family":"Dalmarco","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia Silvia","family":"Fr\u00f6de","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universit\u00e1rio, Trindade, Florian\u00f3polis, 88040-970 SC-, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.02.029"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200102013440507"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00105013"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2006.00784.x"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00022011"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15472.x"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/J.BIOTECHADV.2015.08.001"},{"key":"8","first-page":"98","volume":"78","year":"2008","journal-title":"Gaz M\u00e9dica Da Bahia"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.08.004"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1590\/s0102-695x2005000400020"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1590\/2175-7860201162308"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-50532011001200021"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(01)00420-2"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1515\/znc-2002-3-413"},{"issue":"1","key":"15","first-page":"59","volume":"23","year":"1992","journal-title":"Archives of Medical Research"},{"key":"16","first-page":"1100","volume":"2","year":"2010","journal-title":"Pharmacology"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1250584"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.2307\/2807644"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.2147\/Jep.S37135"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/J.BJP.2015.01.004"},{"key":"21","first-page":"83","volume":"32","year":"1980","journal-title":"Cien Cult"},{"key":"22","first-page":"377","volume":"65","year":"1994","journal-title":"Fitoterapia"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1378\/chest.08-0440"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.05.004"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201100128"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12676"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.03.032"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.5935\/0103-5053.20160186"},{"volume":"4","volume-title":"Adenosine deaminase: colorimetric method","year":"1984","key":"29"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1007\/BF00920477"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90118-X"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1021\/jf026187q"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.05.081"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1021\/jf050046h"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1021\/jf062314x"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2015.1030740"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1080\/09629350123332"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e32833a7154"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1007\/S11882-007-0071-6"},{"key":"41","first-page":"9","volume":"10","year":"2011","journal-title":"Bios"},{"key":"42","first-page":"110","volume":"45","year":"2002","journal-title":"Proceedings of the Western Pharmacology Society"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4783"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.2174\/138945012800564095"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.226845"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-228122"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.2.1254"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2013.6"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1007\/s10068-015-0034-y"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2000.0816"},{"key":"51","first-page":"279","volume":"62","year":"2007","journal-title":"Thorax"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.05.014"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2011.03.005"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/j.aller.2013.11.002"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.10.024"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.11.053"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1165\/Rcmb.2008-0038oc"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2011.06.003"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3794"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200385"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-7-135"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2010.03544.x"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2010.02.018"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058892"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001651"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/352371"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/828521"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.06.004"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2020\/4620251.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2020\/4620251.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2020\/4620251.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T18:31:46Z","timestamp":1579977106000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/mi\/2020\/4620251\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,25]]},"references-count":67,"alternative-id":["4620251","4620251"],"URL":"https:\/\/doi.org\/10.1155\/2020\/4620251","relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"type":"print","value":"0962-9351"},{"type":"electronic","value":"1466-1861"}],"subject":[],"published":{"date-parts":[[2020,1,25]]}}}