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It\u2019s therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, <jats:italic>n<\/jats:italic>\u2010butanol, and water. 500 TCM\u2010simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50\u2019s of the hit fractions range from 1.08 to 6.45\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti\u2010influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re\u2010emerging viruses such as Ebola virus and current SARS\u2010CoV\u20102 pandemic.<\/jats:p>","DOI":"10.1155\/2020\/4979850","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T20:20:11Z","timestamp":1602793211000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Screening for Anti\u2010Influenza Actives of Prefractionated Traditional Chinese Medicines"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9695-839X","authenticated-orcid":false,"given":"Qianli","family":"Kang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5482-2585","authenticated-orcid":false,"given":"Yanyan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinghua","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiqiang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Rong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6261-9202","authenticated-orcid":false,"given":"Rong","family":"Rong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9172-7824","authenticated-orcid":false,"given":"Ruikun","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,15]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2009.00105.x"},{"key":"e_1_2_10_2_2","volume-title":"Influenza","author":"WHO","year":"2018"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.03.014"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67338-2"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc0910060"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5b01055"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4510"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules13061406"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/np9007359"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.2533\/chimia.2007.327"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/v11090826"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/v10060325"},{"key":"e_1_2_10_13_2","article-title":"Screening of neuraminidase inhibitory activities of some medicinal plants traditionally used in lingnan Chinese medicines","volume":"18","author":"Liu J.","year":"2015","journal-title":"Bmc Complementary & Alternative Medicine"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20034290"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/v10120679"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2014.06.005"},{"key":"e_1_2_10_17_2","article-title":"Baloxavir marboxil, a novel cap-dependent endonuclease inhibitor potently suppresses influenza virus replication and represents therapeutic effects in both immunocompetent and immunocompromised mouse models","volume":"14","author":"Fukao K A. 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