{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:33:36Z","timestamp":1773290016177,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T00:00:00Z","timestamp":1578528000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702850"],"award-info":[{"award-number":["81702850"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011841","name":"Xinxiang Medical University","doi-asserted-by":"publisher","award":["505290"],"award-info":[{"award-number":["505290"]}],"id":[{"id":"10.13039\/501100011841","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. The abnormal expression of miR\u2010384 has been acknowledged in the proliferation or metastasis of some cancers. However, the function and the underlying mechanism of miR\u2010384 in PTC progression remain largely unknown. <jats:italic>Methods<\/jats:italic>. Real\u2010time PCR was conducted to detect miR\u2010384 expression in 58 cases of PTC and their adjacent noncancerous tissues. MTT, soft agar assay Transwell assay, and wound\u2010healing assay were carried out to explore the biological function of miR\u2010384 in PTC cell lines of BCPAP and K1. Bioinformatics analysis, dual\u2010luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR\u2010384. Moreover, Spearman\u2019s correlation analysis was conducted to reveal the correlation between miR\u2010384 and PRKACB mRNA in PTC. <jats:italic>Results<\/jats:italic>. The expression of miR\u2010384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size. The ectopic upregulation of miR\u2010384 significantly suppressed PTC progression, and the inhibition of miR\u2010384 had the opposite effects. Moreover, PRKACB gene was confirmed as the target of miR\u2010384. <jats:italic>Conclusion<\/jats:italic>. The study suggests that miR\u2010384 serves as a tumor suppressor in PTC progression by directly targeting the 3\u2032\u2010UTR of PRKACB gene.<\/jats:p>","DOI":"10.1155\/2020\/4983420","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T23:31:09Z","timestamp":1578612669000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["MicroRNA\u2010384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-4200","authenticated-orcid":false,"given":"Yongxia","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beixi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangnan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7435-8078","authenticated-orcid":false,"given":"Xinlai","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7994-2890","authenticated-orcid":false,"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,9]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-015-9724-3"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/a-0859-7454"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.4143\/crt.2015.118"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncpendmet0022"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000369676"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep27086"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/md.0000000000005794"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132821"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2014.59.8391"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.8113"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-015-0189-7"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.09.009"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1379"},{"key":"e_1_2_8_14_2","first-page":"311","article-title":"MicroRNA in colorectal cancer: new perspectives for diagnosis, prognosis and treatment","volume":"22","author":"Bonfrate L.","year":"2013","journal-title":"Journal of Gastrointestinal and Liver Diseases"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.2147\/cmar.s182625"},{"key":"e_1_2_8_16_2","first-page":"1177","article-title":"MicroRNA-338-3p inhibits thyroid cancer progression through targeting AKT3","volume":"7","author":"Sui G. Q.","year":"2017","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.3892\/or.2018.6385"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12704"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-018-0628-6"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12389"},{"key":"e_1_2_8_21_2","first-page":"30","article-title":"Diagnosis and treatment of patients with thyroid cancer","volume":"8","author":"Nguyen Q. T.","year":"2015","journal-title":"American Health and Drug Benefits"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2014.56.8253"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2018.1560203"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1900"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201707779"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2769"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.10.028"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2988"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22819"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm952"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.98296"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/igc.0b013e318259d8da"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.2147\/ott.s167162"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/4983420.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/4983420.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/4983420","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T09:13:46Z","timestamp":1735636426000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/4983420"}},"subtitle":[],"editor":[{"given":"Alfredo","family":"Conti","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/4983420"],"URL":"https:\/\/doi.org\/10.1155\/2020\/4983420","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"4983420"}}