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The aim of this study was to establish the prevalence of <jats:italic>E.coli<\/jats:italic> producing Extended-Spectrum beta-Lactamase (ESBL) antibiotics from rats and gregarious animals in a semirural area of Gabon and to evaluate the origin of a resistance distribution in the environment from animal feces. The bacterial culture was carried out, and the identification of <jats:italic>E. coli<\/jats:italic> strains on a specific medium and the antibiotic susceptibility tests allowed establishing the prevalence. Characterization of resistance genes was performed by gene amplification after DNA extraction. On 161 feces collected in rats, 32 strains were isolated, and 11 strains of <jats:italic>E. coli<\/jats:italic> produced ESBL with a prevalence of 34.37%. Molecular tests showed that CTX-M genes 214\u2009bp were identified in rats. The presence of CTX-M genes could have a human origin. So, the rats can carry ESBL-producing <jats:italic>Enterobacteriaceae<\/jats:italic> which poses a risk to human health and pets in this region of Gabon.<\/jats:p>","DOI":"10.1155\/2020\/5163493","type":"journal-article","created":{"date-parts":[[2020,7,18]],"date-time":"2020-07-18T23:31:09Z","timestamp":1595115069000},"page":"1-6","source":"Crossref","is-referenced-by-count":8,"title":["Prevalence of Extended-Spectrum <i>\u03b2<\/i>-Lactamases in <i>E. coli<\/i> of Rats in the Region North East of Gabon"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0461-4670","authenticated-orcid":true,"given":"Richard","family":"Onanga","sequence":"first","affiliation":[{"name":"Laboratory of Bacteriology, International Medical Research Center of Franceville, P.O. 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