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The difficulties of diagnosis lead to infection rates being underreported. Asymptomatic patients have chronic infections that can lead to severe hyperinfection syndrome or disseminated strongyloidiasis in immunocompromised patients. Strongyloidiasis can easily be misdiagnosed because conventional faecal-based techniques lack of sensitivity for the morphological identification of infective larvae in faeces. None of the currently used molecular methods have used urine samples as an alternative to faecal samples for diagnosing strongyloidiasis. This study was thus aimed at comparing, for the first time, the use of a new loop-mediated isothermal amplification (LAMP) molecular assay (<jats:italic>Strong-<\/jats:italic>LAMP) to traditional methods on patients\u2019 urine samples. Twenty-four urine samples were taken from patients included in a study involving two Spanish hospitals for strongyloidiasis screening using parasitological and serological tests. <jats:italic>Strongyloides<\/jats:italic> larvae were found in 11 patients\u2019 faecal samples, thereby ascertaining that they had the disease. Other patients had high antibody titres but no larvae were found in their faeces. All urine samples were analysed by PCR and <jats:italic>Strong-<\/jats:italic>LAMP assay. No amplification occurred when using PCR. <jats:italic>Strong-<\/jats:italic>LAMP led to detecting <jats:italic>S. stercoralis<\/jats:italic> DNA in urine samples from patients having previously confirmed strongyloidiasis by parasitological tests and\/or a suspicion of being infected by serological ones. The <jats:italic>Strong<\/jats:italic>-LAMP assay is a useful molecular tool for research regarding strongyloidiasis in human urine samples. After further validation, the <jats:italic>Strong<\/jats:italic>-LAMP assay could also be used for complementary and effective diagnosis of strongyloidiasis in a clinical setting.<\/jats:p>","DOI":"10.1155\/2020\/5265198","type":"journal-article","created":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T23:30:56Z","timestamp":1590967856000},"page":"1-10","source":"Crossref","is-referenced-by-count":14,"title":["<i>Strong<\/i>-LAMP Assay Based on a <i>Strongyloides<\/i> spp.-Derived Partial Sequence in the 18S rRNA as Potential Biomarker for Strongyloidiasis Diagnosis in Human Urine Samples"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4089-4307","authenticated-orcid":true,"given":"Pedro","family":"Fern\u00e1ndez-Soto","sequence":"first","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, Salamanca 37007, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6934-3659","authenticated-orcid":true,"given":"Carmen T.","family":"Celis-Giraldo","sequence":"additional","affiliation":[{"name":"Animal Science Faculty, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A), Bogot\u00e1 111166, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coralina","family":"Collar-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, Salamanca 37007, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6470-3894","authenticated-orcid":true,"given":"\u00d3scar","family":"Gorgojo","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, Salamanca 37007, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0246-0716","authenticated-orcid":true,"given":"Milena","family":"Camargo","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Department, Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Bogot\u00e1 111321, Colombia"},{"name":"PhD Programme in Biomedical and Biological Sciences, School of Medicine and Health Sciences, Universidad del Rosario, Bogot\u00e1 112111, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"ISGlobal, Barcelona Ctr. 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