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However, the specific molecular pathways underlying this condition remain unclear. Previous research used next\u2010generation RNA sequencing to identify a series of differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMCs) when compared between patients with AS and healthy controls, thus implying that these DEGs may be related to AS. Furthermore, by screening these DEGS, it may be possible to facilitate clinical diagnosis and optimize treatment strategies. In order to test this hypothesis, we recruited 15 patients with AS and 15 healthy controls. We randomly selected five subjects from each group of patients for RNA sequencing analysis. Sequence reads were generated by an Illumina HiSeq2500 platform and mapped on to the human reference genome using HISAT2. We successfully identified 973 significant DEGs (<jats:italic>p<\/jats:italic> &lt; 0.05) in PBMCs. When compared with controls, 644 of these genes were upregulated (with a fold\u2009change\u2009(FC) &gt; 2) in AS patients and 329 were downregulated (FC &lt; 0.5). Our analysis identified numerous genes related to immune response. Gene Ontology (GO) analysis indicated that these DEGs were significantly related to the positive regulation of epidermal growth factor\u2010activated receptor activity, the positive regulation of the ERBB (erb\u2010b2 receptor tyrosine kinase) signaling pathway, the differentiation of trophoblast giant cells, oxygen transport, immune\u2010related pathways, and inflammation\u2010related pathways. The DEGs were also closely related to the TNF and NF\u2010<jats:italic>\u03ba<\/jats:italic>B signaling pathways. Six DEGs were verified by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR). Receiver operating characteristic (ROC) curve analysis indicated that IL6 may represent a useful biomarker for diagnosing AS. The development of new biomarkers may help us to elucidate the specific mechanisms involved in the development and progression of AS.<\/jats:p>","DOI":"10.1155\/2020\/5304578","type":"journal-article","created":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T23:31:44Z","timestamp":1591659104000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["RNA Sequencing for Gene Expression Profiles in Peripheral Blood Mononuclear Cells with Ankylosing Spondylitis RNA"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9327-8583","authenticated-orcid":false,"given":"Dan","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3101-7553","authenticated-orcid":false,"given":"Jian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxiang","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2183-9430","authenticated-orcid":false,"given":"Hui","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianting","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.14956.1"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2011.150037"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2009.258"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/key239"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.2147\/TACG.S37325"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21192"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2465481"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-018-9523-x"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.7548"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-11-0212"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007071"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2018.79145"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29233"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000477883"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2474-14-354"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280321"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/24.3.242"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.095"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3122"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-3-r25"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1226"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0512-6_3"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(01)00297-2"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01570"},{"key":"e_1_2_9_27_2","unstructured":"VrchotickyA. 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