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The standard curve showed a linear regression relationship with a coefficient of 0.999 and a slope of <jats:italic>y<\/jats:italic>\u2009=\u2009\u22123.249<jats:italic>x<\/jats:italic>\u2009+\u200938.958 corresponding to the amplification efficiency at 99.8%. The limit of the qPCR method was 51.9\u2009copies\/<jats:italic>\u03bc<\/jats:italic>l. The established qPCR method showed excellent specificity, with no cross\u2010reaction observed with common porcine pathogens. The coefficient of variation for intra\u2010assay and interassay variability ranged up to 1.51% and 2.24%, respectively. 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