{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T21:00:40Z","timestamp":1763499640794,"version":"3.41.2"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":351,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860869"],"award-info":[{"award-number":["81860869"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Central precocious puberty (CPP) severely affects children\u2019s physical and mental health and needs to be treated promptly and effectively. This article aimed to research the therapeutic effect of Shugan Xiehuo Formula (SXF) on CPP. A female CPP rat model was established and then treated with leuprolide and different doses of SXF. Sex organ volume and index were measured. Ovaries and uteri were visualized by hematoxylin\u2010eosin staining. The concentrations of follicle\u2010stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and estradiol (E2) in peripheral blood were determined. The expression levels of gonadotropin\u2010releasing hormone (GnRH), gonadotropin\u2010releasing hormone receptor (GnRHR), estrogen receptor alpha (ER<jats:italic>\u03b1<\/jats:italic>), and G protein\u2010coupled receptor 30 (GPR30) in the hypophysis were investigated by Real\u2010Time Quantitative Reverse Transcription PCR and western blot. GnRH expression in the hypothalamus and GnRHR expression in the ovary were detected by immunohistochemistry. SXF reduced the volume of the bilateral ovaries, as well as the volumes of the uterus, hypothalamus, and hypophysis in the female CPP rats and diminished the index of the ovary, uterus, hypothalamus, and hypophysis in the female CPP rats (<jats:italic>P<\/jats:italic> &lt; 0.05 or <jats:italic>P<\/jats:italic> &lt; 0.01). SXF treatment inhibited follicle maturation and uterine wall thickening in the female CPP rats. SXF decreased the concentrations of FSH, LH, PRL, and E2 in the peripheral blood in the female CPP rats (<jats:italic>P<\/jats:italic> &lt; 0.01 or <jats:italic>P<\/jats:italic> &lt; 0.001). SXF suppressed the expressions of GnRH, GnRHR, ER<jats:italic>\u03b1<\/jats:italic>, and GPR30 in the hypophysis (<jats:italic>P<\/jats:italic> &lt; 0.05), the expression of GnRH in the hypothalamus (<jats:italic>P<\/jats:italic> &lt; 0.01), and the expression of GnRHR in the ovaries (<jats:italic>P<\/jats:italic> &lt; 0.001) of the female CPP rats. Overall, our study revealed that SXF had therapeutic effects on CPP in female rats. This is worthy of promoting clinically.<\/jats:p>","DOI":"10.1155\/2020\/5916168","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T01:52:37Z","timestamp":1608256357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Therapeutic Effect of Shugan Xiehuo Formula in the Female Rat Model with Central Precocious Puberty"],"prefix":"10.1155","volume":"2020","author":[{"given":"Weiping","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songmei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dianfei","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6841-1007","authenticated-orcid":false,"given":"Lei","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5759-5891","authenticated-orcid":false,"given":"Jie","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40272-015-0130-8"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2018.05.008"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0201906"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/js.2019-00036"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1515\/jpem-2018-0290"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.4274\/jcrpe.2017.S004"},{"key":"e_1_2_12_7_2","unstructured":"YinW. XiaJ. SuY.et al. Clinical study of 36 cases of idiopathic central precocious puberty treated with shugan xihuo recipe Hebei Traditional Chinese Medicine 37 no. 7 997\u2013998."},{"key":"e_1_2_12_8_2","article-title":"Effect of shuganxiehuo recipe on expressions of gonad, estrogen, GnRH and pituitary GnRH-R in the hypothalamus of female precocious rats","author":"Weiping Y.","year":"2018","journal-title":"Pharmacology and Clinics of Chinese Materia Medica"},{"key":"e_1_2_12_9_2","article-title":"Female reproducti effect of shuganxiehuo recipe on expressions of gonad, estrogen, GnRH and pituitary GnRH-R in the hypothalamus of female precocious rats ve maturation in the absence of kisspeptin\/GPR54 signaling","volume":"34","author":"Mayer C.","year":"2020","journal-title":"Nature Neuroscience"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu009"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4868045"},{"key":"e_1_2_12_12_2","article-title":"Expression of melatonin receptor in the hypothalamus and pituitary of female rats with precocious puberty","author":"Zhou Q.","year":"2013","journal-title":"Acta Universitatis Medicinalis Nanjing"},{"key":"e_1_2_12_13_2","first-page":"386","article-title":"Experimental study on therapeutic effect of Dabuyin Wan on true precocious puberty in female rats","volume":"38","author":"Cheng M.","year":"2013","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.21307\/ane-2018-034"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpag.2014.09.006"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200241070-00003"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1158\/1538-7445.AM2016-944"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-08-0091"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(18)33639-4"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25408"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1515\/jpem-2012-0069"},{"key":"e_1_2_12_22_2","article-title":"GPR30 modulates estrogen-stimulated proliferation of breast and endometrial cancer cells by regulating estrogen receptor alpha homeostasis","volume":"68","author":"Ariazi E.","year":"2008","journal-title":"Cancer Research"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/5916168.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/5916168.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/5916168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T09:17:45Z","timestamp":1723022265000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/5916168"}},"subtitle":[],"editor":[{"given":"Woon-Man","family":"Kung","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/5916168"],"URL":"https:\/\/doi.org\/10.1155\/2020\/5916168","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"type":"print","value":"1741-427X"},{"type":"electronic","value":"1741-4288"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2020-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5916168"}}