{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T20:42:43Z","timestamp":1779309763605,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T00:00:00Z","timestamp":1604102400000},"content-version":"vor","delay-in-days":304,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012829","name":"Guangdong Provincial Hospital of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["YN2016XP03"],"award-info":[{"award-number":["YN2016XP03"]}],"id":[{"id":"10.13039\/100012829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013385","name":"National Science and Technology Program during the Twelfth Five-year Plan Period","doi-asserted-by":"publisher","award":["2013ZX10005002-002"],"award-info":[{"award-number":["2013ZX10005002-002"]}],"id":[{"id":"10.13039\/501100013385","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>The aim of this study was to determine if microRNA (miRNA) expression is different among chronic hepatitis B (CHB) patients with early liver fibrosis classified according to traditional Chinese medicine (TCM) syndromes. Eighteen CHB\u2010fibrosis patients and 12 CHB patients without fibrosis were enrolled. The CHB\u2010fibrosis group included 9 patients with the TCM syndrome of Ganyu Pixu Xueyu (GYPXXY), characterized by liver stagnation, spleen deficiency, and blood stasis, and 9 patients with the TCM syndrome of Qixu Xueyu (QXXY), characterized by deficiency of qi, blood, and blood stasis. Agilent miRNA microarray was performed first in liver specimens to determine whether miRNA expression is different in patients with these two TCM syndromes of CHB\u2010fibrosis. Gene Ontology (GO) analysis and KEGG analysis were applied to determine the roles of the differentially expressed miRNAs. QRT\u2010PCR was performed to validate the Agilent miRNA microarray results. Compared with GYPXXY patients, 6 differentially expressed miRNAs were upregulated (miR\u2010144\u20105p, miR\u201018a\u20105p, miR\u2010148b\u20103p, miR\u2010654\u20103p, miR\u2010139\u20103p, and miR\u201024\u20101\u20105p) and 1 was downregulated (miR\u20106834\u20103p) in QXXY patients. According to qRT\u2010PCR data, miR\u2010144\u20105p and miR\u2010654\u20103p were confirmed as upregulated in CHB\u2010liver fibrosis patients compared to CHB patients without fibrosis, whereas the other 4 miRNAs were not significantly different. More importantly, miR\u2010654\u20103p was confirmed to be significantly upregulated in QXXY patients compared with values in GYPXXY patients, whereas no significant difference was found in miR\u2010144\u20105p. Moreover, the pathways of central carbon metabolism in cancer and cell cycle related to miR\u2010654\u20103p and the target genes of PTEN and ATM were found to be different between QXXY patients and GYPXXY patients. These results indicate that there are different miRNAs, pathways, and target genes between QXXY patients and GYPXXY patients. However, due to the limited sample, whether miR\u2010654\u20103p and the target genes PTEN and ATM could be molecular markers to differentiate TCM syndromes could not be established.<\/jats:p>","DOI":"10.1155\/2020\/5956940","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T17:50:05Z","timestamp":1604166605000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differences in MicroRNA Expression in Chronic Hepatitis B Patients with Early Liver Fibrosis Based on Traditional Chinese Medicine 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