{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T12:32:54Z","timestamp":1768825974914,"version":"3.49.0"},"reference-count":68,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T00:00:00Z","timestamp":1578528000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009509","name":"Kementerian Riset Teknologi Dan Pendidikan Tinggi Republik Indonesia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009509","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Malaria is one of the life\u2010threatening diseases in the world. The spread of resistance to antimalarial drugs is a major challenge, and resistance to artemisinin has been reported in the Southeast Asian region. In the previous study, the active compound of <jats:italic>Streptomyces hygroscopicus<\/jats:italic> subsp. Hygroscopicus (<jats:italic>S<\/jats:italic>. <jats:italic>hygroscopicus<\/jats:italic>), eponemycin, has been shown to have antimalarial effects. To further analyze the effects of other active compounds on the <jats:italic>Plasmodium<\/jats:italic> parasite, identifying and analyzing the effectiveness of compounds contained in <jats:italic>S<\/jats:italic>. <jats:italic>hygroscopicus<\/jats:italic> through instrumentation of liquid chromatography\/mass spectrometry (LC\/MS) and in silico studies were very useful. This study aimed at identifying other derivative compounds from <jats:italic>S<\/jats:italic>. <jats:italic>hygroscopicus<\/jats:italic> and screening the antimalarial activity of the compound by assessing the binding affinity, pharmacokinetic profile, and bond interaction. The derivative compounds were identified using LC\/MS. Protein targets for derivative compounds were found through literature studies, and the results of identification of compounds and protein targets were reconstructed into three\u2010dimensional models. Prediction of pharmacokinetic profiles was carried out using Swiss ADME. Screening of protein targets for the derivative compound was carried out using the reverse molecular docking method. Analyzing bond interaction was done by LigPlot. One compound from <jats:italic>S<\/jats:italic>. <jats:italic>hygroscopicus<\/jats:italic>, i.e., 6,7\u2010dinitro\u20102\u2010[1, 2, 4]triazole\u20104\u2010yl\u2010benzo[de]isoquinoline\u20101,3\u2010dione, was successfully identified using LC\/MS. This compound was an isoquinoline derivative compound. Through literature studies with inclusion criteria, thirteen protein targets were obtained for reverse molecular docking. This isoquinoline derivative had the potential to bind to each protein target. The pharmacokinetic profile showed that this compound had the drug\u2010likeness criteria. <jats:italic>Conclusion<\/jats:italic>. 6,7\u2010Dinitro\u20102\u2010[1, 2, 4]triazole\u20104\u2010yl\u2010benzo[de]isoquinoline\u20101,3\u2010dione has antimalarial activity as shown by reverse molecular docking studies and pharmacokinetic profiles. The best inhibitory ability of compounds based on bond affinity is with adenylosuccinate synthetase.<\/jats:p>","DOI":"10.1155\/2020\/6135696","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T23:31:24Z","timestamp":1578612684000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Antimalarial Properties of Isoquinoline Derivative from <i>Streptomyces hygroscopicus<\/i> subsp. Hygroscopicus: An In Silico Approach"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4314-6961","authenticated-orcid":false,"given":"Rivo YB.","family":"Nugraha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6767-5577","authenticated-orcid":false,"given":"Icha FD.","family":"Faratisha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0972-2918","authenticated-orcid":false,"given":"Kana","family":"Mardhiyyah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5015-8100","authenticated-orcid":false,"given":"Dio G.","family":"Ariel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4535-3331","authenticated-orcid":false,"given":"Fitria F.","family":"Putri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-9282","authenticated-orcid":false,"family":"Nafisatuzzamrudah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sri","family":"Winarsih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4580-913X","authenticated-orcid":false,"given":"Teguh W.","family":"Sardjono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4880-1048","authenticated-orcid":false,"given":"Loeki E.","family":"Fitri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,9]]},"reference":[{"key":"e_1_2_8_1_2","first-page":"9","article-title":"A study of drug resistance in malaria","volume":"15","author":"Melmane P.","year":"2014","journal-title":"JIACM"},{"key":"e_1_2_8_2_2","unstructured":"World Health Organization (WHO) World malaria report summary 2016 WHO Geneva Switzerland."},{"key":"e_1_2_8_3_2","unstructured":"DepkesR. 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