{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T08:06:14Z","timestamp":1790409974866,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T00:00:00Z","timestamp":1581984000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advances in Virology"],"published-print":{"date-parts":[[2020,2,18]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    . In Morocco, pediatric pneumonia remains a serious public health problem, as it constitutes the first cause of mortality due to infectious diseases. The etiological diagnosis of acute respiratory tract infections is difficult. Therefore, it is necessary to use Multiplex real-time polymerase chain reaction assay tests in a routine setting for exact and fast identification.\n                    <jats:italic>Objectives<\/jats:italic>\n                    . In this paper, we present the clinical results of pediatric pneumonia and describe their etiology by using molecular diagnosis.\n                    <jats:italic>Study design<\/jats:italic>\n                    : Tracheal secretion was collected from infants presenting respiratory distress isolated or associated with systemic signs, attending the unit of Neonatology between December 1, 2016, and Mai 31, 2018. Samples were tested with the multiplex RespiFinder\u00ae SMART 22 FAST which potentially detects 18 viruses and 4 bacteria.\n                    <jats:italic>Results<\/jats:italic>\n                    . Of the 86 infants considered in this study (mean age 31\u2009\u00b1\u200919 days) suspected of acute respiratory tract infections, 71 (83%) were positive for one or multiple viruses or\/and bacteria. The majority of acute respiratory tract infections had a viral origin (95%): respiratory syncytial viruses (A and B) (49%), rhinovirus (21%), coronaviruses 229E (11%), humain metapneumovirus (5%), influenza A (3%), influenza H1N1 (1%), adenovirus (2%), and parainfluenza virus type 4 (2%). Among our patients, 6% had\n                    <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    . Coinfections were not associated with severe respiratory symptoms.\n                    <jats:italic>Conclusion<\/jats:italic>\n                    . The clinical spectrum of respiratory infections is complex and often nonspecific. Thus, the early and fast detection of related causative agents is crucial. The use of multiplex real time polymerase chain reaction may help choose an accurate treatment, reduce the overall use of unnecessary antibiotics, preserve intestinal flora, and decrease nosocomial infection by reducing the length of hospitalization.\n                  <\/jats:p>","DOI":"10.1155\/2020\/6212643","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T18:31:42Z","timestamp":1582050702000},"page":"1-7","source":"Crossref","is-referenced-by-count":10,"title":["Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder\u00ae SMART 22 FAST in a Group of Moroccan Infants"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4870-191X","authenticated-orcid":true,"given":"Kenza","family":"Hattoufi","sequence":"first","affiliation":[{"name":"National Reference Center in Neonatology and Nutrition, Children's Hospital, University Hospital Centre IBN SINA, Rabat, Morocco"},{"name":"Research Team 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Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aicha","family":"Kharbach","sequence":"additional","affiliation":[{"name":"Research Team on Health and Nutrition of Mother and Child, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco"},{"name":"Gynaecology-Obstetrics and Endocrinology Department, Maternity Souissi, University Hospital Center IBN SINA, University Mohammed V, Rabat, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5724-2751","authenticated-orcid":true,"given":"Amina","family":"Barkat","sequence":"additional","affiliation":[{"name":"National Reference Center in Neonatology and Nutrition, Children's Hospital, University Hospital Centre IBN SINA, Rabat, Morocco"},{"name":"Research Team on Health and Nutrition of Mother and Child, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, 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