{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:08:29Z","timestamp":1785190109018,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T00:00:00Z","timestamp":1585094400000},"content-version":"vor","delay-in-days":84,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Excessive alcohol consumption is a worldwide threat with severe morbidity and mortality. Other than abstinence, there is still no FDA\u2010approved drug for alcoholic liver disease (ALD). Liver is the primary site of ethanol metabolism and hence gets the most damage from excessive drinking. It triggers multiple signalling events including inflammation, leading to an array of hepatic lesions like steatosis, hepatitis, fibrosis, and cirrhosis. Similarly, when medications or xenobiotic compounds are ingested orally, the liver gets the highest exposure of those metabolites, which in turn can cause severe liver toxicity. BV\u20107310 is a standardized mixture of four Ayurvedic plants, namely, <jats:italic>Phyllanthus niruri, Tephrosia purpurea<\/jats:italic>, <jats:italic>Boerhavia diffusa,<\/jats:italic> and <jats:italic>Andrographis paniculata.<\/jats:italic> In different systems of traditional medicine, each of these plants has been known to have use in gastrointestinal disorders. We wanted to assess the combined effect of these plant extracts on alcohol\u2010induced liver damage. First, we investigated the hepatoprotective activity of BV\u20107310 against alcohol\u2010induced toxicity in human liver HepG2 cells. Ethanol treatment (120\u2009mM for 48 hours) significantly showed toxicity (around 42%) in these cells, and coincubation with BV\u20107310 prevented ethanol\u2010induced cell death in a dose\u2010dependent manner. Interestingly, the formulation BV\u20107310 showed synergistic activity than any individual extract tested in this assay. BV\u20107310 also showed potent antioxidant activity in 2,2\u2010diphenyl\u20101\u2010picryl\u2010hydrazyl (DPPH) assay. Next, we induced hepatitis in Sprague\u2013Dawley (SD) rats using repeated alcohol (40%) dosing, and carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) 24 hours before termination. Both oral doses of BV\u20107310 (250 and 500\u2009mg\/kg body weight) protected the alcohol\u2010induced body weight loss and significantly improved the elevated levels of liver enzymes compared to the vehicle treated group. Thus, BV\u20107310 prevents alcohol\u2010induced toxicity in both in\u2010vitro and in\u2010vivo models and could be beneficial for the treatment of ALD or other conditions, which may cause liver toxicity.<\/jats:p>","DOI":"10.1155\/2020\/6428906","type":"journal-article","created":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T23:30:52Z","timestamp":1585179052000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Hepatoprotective Activity of BV\u20107310, a Proprietary Herbal Formulation of <i>Phyllanthus niruri<\/i>, <i>Tephrosia purpurea<\/i>, <i>Boerhavia diffusa,<\/i> and <i>Andrographis paniculata<\/i>, in Alcohol\u2010Induced HepG2 Cells and Alcohol plus a Haloalkane, CCl<sub>4<\/sub>, Induced Liver Damage in Rats"],"prefix":"10.1155","volume":"2020","author":[{"given":"Debendranath","family":"Dey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6488-2079","authenticated-orcid":false,"given":"Sunetra","family":"Chaskar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9226-3251","authenticated-orcid":false,"given":"Narendra","family":"Bhatt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9392-4709","authenticated-orcid":false,"given":"Deepa","family":"Chitre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,25]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cotox.2018.10.003"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/713611034"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/10590500701569398"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.13593"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28464"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0122124"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020635"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD002800"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.cd002800.pub2"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.01.057"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6171"},{"key":"e_1_2_8_12_2","article-title":"Pharmacological interventions for alcoholic liver disease (alcohol-related liver disease): an attempted network meta-analysis","volume":"3","author":"Buzzetti E.","year":"2017","journal-title":"Cochrane Database Systematic Reviews"},{"key":"e_1_2_8_13_2","first-page":"284","article-title":"Hepato-protective activity of andrographolide against galactosamine and paracetamol intoxication in rats","volume":"92","author":"Handa S. 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