{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:32:55Z","timestamp":1771655575003,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"vor","delay-in-days":364,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004317","name":"Shihezi University","doi-asserted-by":"publisher","award":["ZZZC201921A"],"award-info":[{"award-number":["ZZZC201921A"]}],"id":[{"id":"10.13039\/501100004317","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31760291"],"award-info":[{"award-number":["31760291"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>MicroRNAs (miRNAs) play crucial roles in the development of essential hypertension (EH). Previously, we found that the expression of miR\u20101929\u20103p was decreased in C57BL\/6 mice with hypertension induced by murine cytomegalovirus (MCMV). In this study, we explored the role of miR\u20101929\u20103p in hypertension myocardial remodeling in MCMV\u2010infected mice. First, we measured MCMV DNA and host IgG and IgM after infection and determined the expression of miR\u20101929\u20103p and its target gene endothelin A receptor (Ednra) mRNA in the myocardium of mice. Then, we performed invasive blood pressure (BP) monitoring. Heart\u2010to\u2010body weight ratio (HW\/BW%), along with mRNA levels of B\u2010type natriuretic peptide (BNP) and beta myosin heavy chain (<jats:italic>\u03b2<\/jats:italic>\u2010MHC), revealed myocardial remodeling. Hematoxylin\/eosin and Masson\u2019s trichrome staining indicated morphological changes in the myocardium. Cardiac function was assessed via echocardiography. Moreover, MCMV\u2010infected mice were injected with recombinant adeno\u2010associated virus\u2010 (rAAV\u2010) miR\u20101929\u20103p overexpression vector. Immunohistochemistry and western blotting showed the expression of Ednra and the activation of NOD\u2010like receptor pyrin domain containing 3 (NLRP3) inflammasome. And enzyme\u2010linked immunosorbent assay (ELISA) revealed the concentrations of endothelin\u20101 (ET\u20101), interleukin\u20101<jats:italic>\u03b2<\/jats:italic> (IL\u20101<jats:italic>\u03b2<\/jats:italic>), and interleukin\u201018 (IL\u201018). In this study, we found that decreased expression of miR\u20101929\u20103p in MCMV\u2010infected mice induced high BP and further development of myocardial remodeling cardiac function injury through increased expression of Ednra. Strikingly, overexpression of miR\u20101929\u20103p ameliorated these pathological changes of the heart. The positive effect was shown to be associated with inhibition of NLRP3 inflammasome activation and decreased expression of key proinflammatory cytokine IL\u20101<jats:italic>\u03b2<\/jats:italic>. Collectively, these results indicate that miR\u20101929\u20103p overexpression may effectively alleviate EH myocardial remodeling by suppressing Ednra\/NLRP3 inflammasome activation in MCMV\u2010infected mice.<\/jats:p>","DOI":"10.1155\/2020\/6653819","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T01:20:06Z","timestamp":1609377606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["miR\u20101929\u20103p Overexpression Alleviates Murine Cytomegalovirus\u2010Induced Hypertensive Myocardial Remodeling by Suppressing Ednra\/NLRP3 Inflammasome Activation"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9454-1008","authenticated-orcid":false,"given":"YongJia","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0737-9725","authenticated-orcid":false,"given":"Zhen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7049-4590","authenticated-orcid":false,"given":"Hua","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4854-6195","authenticated-orcid":false,"given":"LaMei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1009-363X","authenticated-orcid":false,"given":"DongMei","family":"Xi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7853-3593","authenticated-orcid":false,"given":"YunZhong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3471-5288","authenticated-orcid":false,"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1180-902X","authenticated-orcid":false,"given":"YongMin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6304-253X","authenticated-orcid":false,"given":"Na","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6912-6187","authenticated-orcid":false,"given":"Fang","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,30]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31919-5"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199816040-00001"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.3.329"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.51"},{"key":"e_1_2_10_5_2","first-page":"1498","article-title":"Variants of tumor necrosis factor-induced protein 3 gene are associated with left ventricular hypertrophy in hypertensive patients","volume":"124","author":"Xue H.","year":"2011","journal-title":"Chinese Medical Journal"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2136652100"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115861108"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.655"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000084505.54603.C7"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcw026"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2014.03.015"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2015.09.035"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1515\/bmc-2016-0017"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.012237"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-017-0663-9"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6448"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.007"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2019.09.055"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20202387"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/10826060701885704"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000427"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101255"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/10641963.2016.1267189"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9080607"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.150874"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-014-9716-2"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1177\/1535370219854325"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.12659\/msm.892861"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0181440"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20171522"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-58680-6"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8060156"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2017.07.001"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108977"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0176-3"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568006054553390"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9090790"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2019.07.012"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045186"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002452"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(00)01178-X"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2020.104788"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/6653819.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/6653819.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/6653819","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T11:33:37Z","timestamp":1735644817000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/6653819"}},"subtitle":[],"editor":[{"given":"Koichiro","family":"Wada","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/6653819"],"URL":"https:\/\/doi.org\/10.1155\/2020\/6653819","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2020-10-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6653819"}}