{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T19:02:59Z","timestamp":1770490979504,"version":"3.49.0"},"reference-count":67,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T00:00:00Z","timestamp":1602201600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Talent Training Program of Guangdong Province Joint Training Graduate Demonstration Base","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"Talent Training Program of Guangdong Province Joint Training Graduate Demonstration Base","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]},{"DOI":"10.13039\/501100008366","name":"Guangdong Pharmaceutical University","doi-asserted-by":"publisher","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}],"id":[{"id":"10.13039\/501100008366","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008366","name":"Guangdong Pharmaceutical University","doi-asserted-by":"publisher","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}],"id":[{"id":"10.13039\/501100008366","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010226","name":"Department of Education of Guangdong Province","doi-asserted-by":"publisher","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010226","name":"Department of Education of Guangdong Province","doi-asserted-by":"publisher","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Laboratory of Bioengineering Drugs of Guangdong Province of China","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"Key Laboratory of Bioengineering Drugs of Guangdong Province of China","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]},{"name":"Swan Project Innovation Team Plan","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"Swan Project Innovation Team Plan","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]},{"name":"Introduction of Leading Talents Program","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"Introduction of Leading Talents Program","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]},{"name":"Tianhe District Science and Technology Plan Project-Key Technologies Project","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"Tianhe District Science and Technology Plan Project-Key Technologies Project","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]},{"name":"National Engineering Research Center for Modernazation of Traditional Chinese Medicine-Sub Center of Artemisiae Argyi Folium","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"National Engineering Research Center for Modernazation of Traditional Chinese Medicine-Sub Center of Artemisiae Argyi Folium","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]},{"name":"Science and Technology Projects of Guangdong Province","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"Science and Technology Projects of Guangdong Province","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]},{"name":"Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System","award":["2018BY003"],"award-info":[{"award-number":["2018BY003"]}]},{"name":"Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System","award":["2016A040402033"],"award-info":[{"award-number":["2016A040402033"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2020,10,9]]},"abstract":"<jats:p>The Chinese herbal medicine, Huzhen Tongfeng Formula (HZTF), derived from traditional Chinese medicine (TCM) practice, has recognized therapeutic benefits for gouty arthritis (GA). HZTF is currently in the late stage of approval process as a new anti-GA drug application. However, the underlying mechanism of HZTF as an antigout medication is unclear. In this study, we combined network pharmacology and experimental validation approaches to elucidate the mechanism of action of HZTF. First, the relative drug-disease target networks were constructed and analyzed for pathway enrichment. Potential pathways were then validated by <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> experiments. We found that 34 compounds from HZTF matched 181 potential drug targets. Topology analysis revealed 77 core targets of HZTF, which were highly related to gout, following screening of KEGG pathway enrichment. Further analysis demonstrated that the arachidonic acid metabolic pathway was the most relevant pathway involved in the mechanism of HZTF. Validation experiments showed that HZTF significantly inhibited the inflammatory cell infiltration into gouty joints, improved the swelling of affected joints, and increased the pain threshold. HZTF significantly reduced the transcription and production of various cytokines and inflammatory mediators <jats:italic>in vitro<\/jats:italic>. In particular, cyclooxygenase (COX)-1, COX-2, and 5-lipoxygenase were simultaneously downregulated. In conclusion, our study suggests that the antigout mechanism of HZTF is associated with the inhibition of the arachidonic acid pathway, resulting in the suppression of inflammatory cytokines and mediators. These findings extend our understanding of the pharmacological action of HZTF, rationalizing the application HZTF as an effective herbal therapy for GA.<\/jats:p>","DOI":"10.1155\/2020\/6950206","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T21:20:06Z","timestamp":1602278406000},"page":"1-17","source":"Crossref","is-referenced-by-count":11,"title":["Chinese Medicine Huzhen Tongfeng Formula Effectively Attenuates Gouty Arthritis by Inhibiting Arachidonic Acid Metabolism and Inflammatory Mediators"],"prefix":"10.1155","volume":"2020","author":[{"given":"Jianping","family":"Deng","sequence":"first","affiliation":[{"name":"Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Department of Pharmaceutics, Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou, China"},{"name":"Guangzhou Jinan Biomedical Research and Development Center, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8472-3016","authenticated-orcid":true,"given":"Zicong","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Department of Pharmaceutics, Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou, China"},{"name":"Guangzhou Jinan Biomedical Research and Development Center, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Department of Pharmaceutics, Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenling","family":"Zhao","sequence":"additional","affiliation":[{"name":"Guangzhou Jinan Biomedical Research and Development Center, Guangzhou, China"},{"name":"College of Life Science and Technology, Jinan University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifei","family":"Li","sequence":"additional","affiliation":[{"name":"Guangzhou Jinan Biomedical Research and Development Center, Guangzhou, China"},{"name":"College of Life Science and Technology, Jinan University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengquan","family":"Su","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Department of Pharmaceutics, Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4637-9701","authenticated-orcid":true,"given":"Yiguang","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9918-2865","authenticated-orcid":true,"given":"Yifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangzhou Jinan Biomedical Research and Development Center, Guangzhou, China"},{"name":"College of Life Science and Technology, Jinan University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2367-0491","authenticated-orcid":true,"given":"Zhiping","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Department of Pharmaceutics, Guangdong Engineering Research Center of Natural Products and New Drugs, Guangdong Provincial University Engineering Technology Research Center of Natural Products and Drugs, Guangdong Pharmaceutical University, Guangzhou, China"},{"name":"Guangzhou Jinan Biomedical Research and Development Center, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.07.024"},{"issue":"2","key":"2","first-page":"90","volume":"14","year":"2017","journal-title":"Iranian journal of immunology."},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.91"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2016.08.006"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.12156"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.7326\/M16-0570"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2017.05.026"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c7086"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60799-0"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.2014.895325"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8591349"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s40264-014-0250-z"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.111917"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.12.004"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.04.042"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-017-0384-6"},{"issue":"12","key":"17","first-page":"1603","volume":"33","year":"2013","journal-title":"Zhongguo Zhong Xi Yi Jie He Za Zhi"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/BF02835742"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/6037037"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.9165"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-008-0103-7"},{"key":"22","year":"2006"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108994"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-019-3064-0"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00811"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.03.035"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.02.004"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw937"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01318"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm415"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(87)90072-8"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.01.035"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2006.10.009"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122245999"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20046888"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-018-0513-x"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.01.011"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00230"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00299"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.10.007"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1097\/shk.0000000000001181"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.104348"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.153036"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1126\/science.6301011"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100943200"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.145"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00346-9"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1126\/science.294.5548.1871"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2017.04.011"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2018.09.005"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-018-1137-8"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.17660\/actahortic.2009.841.28"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.03.055"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22071124"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1016\/j.plefa.2003.11.006"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2019.03.038"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070689"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.152831"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1023\/A:1027322304880"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-013-0172-x"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi.127.749"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04947-6"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.k2893"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.1881"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-13-0083"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1021\/np500796f"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2020\/6950206.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2020\/6950206.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2020\/6950206.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,11]],"date-time":"2020-10-11T12:55:46Z","timestamp":1602420946000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/mi\/2020\/6950206\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,9]]},"references-count":67,"alternative-id":["6950206","6950206"],"URL":"https:\/\/doi.org\/10.1155\/2020\/6950206","relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,9]]}}}