{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T20:35:59Z","timestamp":1780432559365,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000},"content-version":"vor","delay-in-days":342,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004605","name":"Zhengzhou University","doi-asserted-by":"publisher","award":["YNQN 2017037"],"award-info":[{"award-number":["YNQN 2017037"]}],"id":[{"id":"10.13039\/501100004605","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010950","name":"Science and Technology Department, Henan Province","doi-asserted-by":"publisher","award":["182102310512"],"award-info":[{"award-number":["182102310512"]}],"id":[{"id":"10.13039\/501100010950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010950","name":"Science and Technology Department, Henan Province","doi-asserted-by":"publisher","award":["192102310118"],"award-info":[{"award-number":["192102310118"]}],"id":[{"id":"10.13039\/501100010950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010950","name":"Science and Technology Department, Henan Province","doi-asserted-by":"publisher","award":["192102310111"],"award-info":[{"award-number":["192102310111"]}],"id":[{"id":"10.13039\/501100010950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902253"],"award-info":[{"award-number":["81902253"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>We recently reported that necroptosis contributed to compression\u2010induced nucleus pulposus (NP) cells death. In the current study, we investigated the regulative effect of necroptosis inhibitor Necrostatin\u20101 on NP cells apoptosis and autophagy. Necrostatin\u20101, autophagy inhibitor 3\u2010Methyladenine and apoptosis inhibitor Z\u2010VAD\u2010FMK were employed, and NP cells were exposed to 1.0\u2009MPa compression for 0, 24 and 36\u2009h. Necroptosis\u2010associated molecules were measured by Western blot and RT\u2010PCR. Autophagy and apoptosis levels were evaluated by Western blot and quantified by flow cytometry after monodansylcadaverine and Annexin V\u2010FITC\/propidium iodide staining, respectively. The cell viability and cell death were also examined. Furthermore, we measured mitochondrial membrane potential (MMP), mitochondrial permeability transition pore (MPTP) and indices of oxidative stress to assess mitochondrial dysfunction. The results established that Necrostatin\u20101 blocked NP cells autophagy, and 3\u2010Methyladenine had little influence on NP cells necroptosis. The Necrostatin\u20101+3\u2010Methyladenine treatment exerted almost the same role as Necrostatin\u20101 in reducing NP cells death. Necrostatin\u20101 restrained NP cells apoptosis, while Z\u2010VAD\u2010FMK enhanced NP cells necroptosis. The Necrostatin\u20101+Z\u2010VAD\u2010FMK treatment provided more prominent role in blocking NP cells death compared with Necrostatin\u20101, consistent with increased MMP, reduced opening of MPTP and oxidative stress. In summary, the synergistic utilization of Necrostatin\u20101 and Z\u2010VAD\u2010FMK is a very worthwhile solution in preventing compression\u2010mediated NP cells death, which might be largely attributed to restored mitochondrial function.<\/jats:p>","DOI":"10.1155\/2020\/6976317","type":"journal-article","created":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T07:36:22Z","timestamp":1607499382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Synergistic Utilization of Necrostatin\u20101 and Z\u2010VAD\u2010FMK Efficiently Promotes the Survival of Compression\u2010Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction"],"prefix":"10.1155","volume":"2020","author":[{"given":"Songfeng","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfeng","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guowei","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhui","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Kou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1362-9698","authenticated-orcid":false,"given":"Shitao","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3346-9193","authenticated-orcid":false,"given":"Hongjian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.spinee.2017.10.008"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-204428"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-200002150-00016"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.12.010"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.23751"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000001012"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22533"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2284-8"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-200212010-00002"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3999"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-015-1128-y"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.02.003"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature20558"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.06.019"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-018-1455-x"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-017-1358-2"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S140526"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.113815"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.07.007"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.10.034"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-05-2160"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.06.012"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.13747"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1147670"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20170558"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2017.1386359"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.4161\/auto.7.6.15103"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.312561.118"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1616829114"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-018-0166-8"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716578115"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.462341"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2017.07.020"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.12.077"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5678548"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.94"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.06.111"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.05.014"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/6976317.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/6976317.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/6976317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T09:20:24Z","timestamp":1735636824000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/6976317"}},"subtitle":[],"editor":[{"given":"Yu Sheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/6976317"],"URL":"https:\/\/doi.org\/10.1155\/2020\/6976317","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2020-08-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6976317"}}