{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:07:35Z","timestamp":1785434855001,"version":"3.56.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T00:00:00Z","timestamp":1591747200000},"content-version":"vor","delay-in-days":161,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Extended\u2010spectrum <jats:italic>\u03b2<\/jats:italic>\u2010lactamase and their molecular mechanism in <jats:italic>Enterobacteriaceae<\/jats:italic> were analyzed in 126 fish samples of 9 various wild species, living in the lagoon of Bizerte in Tunisia. Fifty\u2010nine (59) Gram\u2010negative strains were isolated and identified as <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>n<\/jats:italic> = 24), <jats:italic>Klebsiella pneumonia<\/jats:italic> (<jats:italic>n<\/jats:italic> = 21), <jats:italic>Citrobacter freundii<\/jats:italic> (<jats:italic>n<\/jats:italic> = 8), and <jats:italic>Shigella boydii<\/jats:italic> (<jats:italic>n<\/jats:italic> = 6). Forty\u2010seven ESBL producers were identified using the synergic test. <jats:italic>\u03b2<\/jats:italic>\u2010Lactamase genes detected were <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-1<\/jats:sub> (<jats:italic>E. coli<\/jats:italic>\/15; <jats:italic>K. pneumonia<\/jats:italic>\/8; <jats:italic>C. freundii<\/jats:italic>\/1; <jats:italic>Sh. boydii<\/jats:italic>\/1), <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-1<\/jats:sub>+ <jats:italic>bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub> (<jats:italic>E. coli<\/jats:italic>\/4; <jats:italic>K. pneumonia<\/jats:italic>\/3), <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-1<\/jats:sub>+ <jats:italic>bla<\/jats:italic><jats:sub>TEM-1-a<\/jats:sub> (<jats:italic>K. pneumonia<\/jats:italic>\/2), <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub>+ <jats:italic>bla<\/jats:italic><jats:sub>TEM-1-a<\/jats:sub> (<jats:italic>K. pneumonia<\/jats:italic>\/1; <jats:italic>Sh. boydii<\/jats:italic>\/1), <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub>+ <jats:italic>bla<\/jats:italic><jats:sub>OXA-1<\/jats:sub> (<jats:italic>K. pneumonia<\/jats:italic>\/1), <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> (<jats:italic>E. coli<\/jats:italic>\/3; <jats:italic>K. pneumonia<\/jats:italic>\/1; <jats:italic>Sh. boydii<\/jats:italic>\/3), and <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-9<\/jats:sub> (<jats:italic>C. freundii<\/jats:italic>\/3). Most strains (84.7%) showed a multiresistant phenotype. <jats:italic>qnrA<\/jats:italic> and <jats:italic>qnrB<\/jats:italic> genes were identified in six <jats:italic>E. coli<\/jats:italic> and in ten <jats:italic>E. coli<\/jats:italic>+one <jats:italic>K. pneumonia<\/jats:italic> isolates, respectively. The resistance to tetracycline and sulfonamide was conferred by the <jats:italic>tet<\/jats:italic> and <jats:italic>sul<\/jats:italic> genes. Characterization of phylogenic groups in <jats:italic>E. coli<\/jats:italic> isolates revealed phylogroups D (<jats:italic>n<\/jats:italic> = 20 strains), B2 (<jats:italic>n<\/jats:italic> = 2), and A (<jats:italic>n<\/jats:italic> = 2). The studied virulence factor showed prevalence of <jats:italic>fimA<\/jats:italic> genes in 9 <jats:italic>E. coli<\/jats:italic> isolates (37.5%). Similarly, no strain revealed the three other virulence factors tested (<jats:italic>eae<\/jats:italic>, <jats:italic>aer<\/jats:italic>, and <jats:italic>cnf1<\/jats:italic>). Our findings confirmed that the lagoons of Bizerte may be a reservoir of multidrug resistance\/ESBL\u2010producing <jats:italic>Enterobacteriaceae<\/jats:italic>. This could lead to indisputable impacts on human and animal health, through the food chain.<\/jats:p>","DOI":"10.1155\/2020\/7132812","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T23:37:49Z","timestamp":1591832269000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Detection of Extended\u2010Spectrum <i>\u03b2<\/i>\u2010Lactamases (ESBL) Producing Enterobacteriaceae from Fish Trapped in the Lagoon Area of Bizerte, Tunisia"],"prefix":"10.1155","volume":"2020","author":[{"given":"Bilel","family":"Hassen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-6727","authenticated-orcid":false,"given":"Ahlem","family":"Jouini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monia","family":"Elbour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safa","family":"Hamrouni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderrazek","family":"Maaroufi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,10]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2009.04.020"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2017.04.046"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.09.025"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2017.0149"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103865"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(01)00317-X"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2008.11.086"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2008.12.006"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005995217901"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/es001069"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolecon.2006.10.022"},{"key":"e_1_2_10_12_2","first-page":"10","volume-title":"Indicators of Stress in the Marine Benthos, IOC Workshop Report No. 195","author":"Warwick R.","year":"2005"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-013-0187-2"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540110080034"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01867.x"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(98)00105-9"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2009.00175.x"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10646-016-1664-4"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0137250"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-015-4105-7"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-009-9585-x"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2014.05.002"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm316"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.2.697-703.1991"},{"key":"e_1_2_10_25_2","volume-title":"Informational Supplement, M100-S, CLSI","author":"Clinical and Laboratory Standards Institute","year":"2017"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3996-4001.2004"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.66.10.4555-4558.2000"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.40.12.4445-4449.2002"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2012.717712"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00054-13"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165820"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2017.04.073"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0572"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1100\/tsw.2006.181"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.3394\/0380-1330(2008)34[228:SASOEC]2.0.CO;2"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2012.09.010"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148135"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2016.01.042"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms5030053"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2015.02.023"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01412-06"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2016.09.009"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2016.10.031"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2016.03.054"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-12-251"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/7132812.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/7132812.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/7132812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T11:24:12Z","timestamp":1735644252000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/7132812"}},"subtitle":[],"editor":[{"given":"Gy\u00f6rgy","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/7132812"],"URL":"https:\/\/doi.org\/10.1155\/2020\/7132812","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"7132812"}}