{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T09:21:53Z","timestamp":1775640113375,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T00:00:00Z","timestamp":1578009600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2020,1,3]]},"abstract":"<jats:p>The aim of the study was to evaluate the clinical significance of microRNA-375 in acute myocardial infarction patients and its mimic action in hypoxia\/reoxygenation- (H\/R-) induced ventricular cardiomyocyte H9c2 injury. In the current study, 90 ST-elevated acute MI patients (STEMI), 75 non-ST-elevated acute MI patients (NSTEMI), 90 healthy subjects, 14 weeks old mice, and ventricular cardiomyocyte H9c2 were included. The expressions of plasma microRNA-375 in patients with STEMI and NSTEMI and AMI mouse models were remarkably decreased than in controls (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>). The areas under the curve (AUC) of plasma microRNA-375 were revealed 0.939 in STEMI and 0.935 in NSTEMI subjects. Moreover, microRNA-375 levels in H\/R-exposed cardiac H9c2 cells were evidently downregulated and significantly increased apoptosis rate and caspase-3 activity levels, while overexpression of miR-375 remarkably reduced apoptosis percentage and caspase-3 levels as compared with normal cells. Furthermore, this study also demonstrated that Nemo-like kinase (NLK), NLK mRNA, and protein expression levels were significantly downregulated in H\/R-injured H9c2 cells, on the contrary, H9c2 cells transfected with mimic-miR-375 greatly upregulated NLK mRNA and protein expression. Plasma microRNA-375 may serve as an essential clinical biomarker for diagnosis of early-stage AMI. Mimic expression of miR-375 significantly prevented H\/R-induced cardiomyocyte injury by decreasing caspase-3 activity through upregulation of the NLK gene, recommended as a new therapeutic option for AMI patient.<\/jats:p>","DOI":"10.1155\/2020\/7164069","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T18:31:55Z","timestamp":1578076315000},"page":"1-10","source":"Crossref","is-referenced-by-count":18,"title":["Overexpression of miR-375 Protects Cardiomyocyte Injury following Hypoxic-Reoxygenation Injury"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7187-6550","authenticated-orcid":true,"given":"Md Sayed","family":"Ali Sheikh","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Cardiology, College of Medicine, Jouf University, Sakaka, Al Jouf, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000659"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20140663"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.hlc.2014.01.001"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.4103\/jfmpc.jfmpc_297_18"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7018393"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28277"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08895-7"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.07.352"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28723"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.01.165"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1159\/000489686"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.12423"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.5152\/AnatolJCardiol.2015.6603"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.78"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.02.087"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1159\/000447825"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0164897"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e31826e1058"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2014.09.011"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3322"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5893064"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27274"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehq013"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2018.07.129"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvy231"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108894"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1957920"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.116.004387"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.2174\/1381612822666160210143047"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.9011"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08989-2"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1161\/circgenetics.114.000968"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2121"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvx052"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/378484"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7164069.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7164069.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7164069.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T18:31:56Z","timestamp":1578076316000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/omcl\/2020\/7164069\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,3]]},"references-count":35,"alternative-id":["7164069","7164069"],"URL":"https:\/\/doi.org\/10.1155\/2020\/7164069","relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,3]]}}}