{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T01:28:35Z","timestamp":1783128515657,"version":"3.54.6"},"reference-count":69,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T00:00:00Z","timestamp":1579910400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004725","name":"Ministry of Economic Affairs","doi-asserted-by":"publisher","award":["108-EC-17-A-22-0318"],"award-info":[{"award-number":["108-EC-17-A-22-0318"]}],"id":[{"id":"10.13039\/501100004725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2020,1,25]]},"abstract":"<jats:p>Cisplatin chemotherapy causes myelosuppression and often limits treatment duration and dose escalation in patients. Novel approaches to circumvent or lessen myelotoxicity may improve clinical outcome and quality of life in these patients. <jats:italic>Chlorella sorokiniana<\/jats:italic> (CS) is a freshwater unicellular green alga and exhibits encouraging efficacy in immunomodulation and anticancer in preclinical studies<jats:italic>.<\/jats:italic> However, the efficacy of CS on chemoprotection remains unclear. We report here, for the first time, that CS extract (CSE) could protect normal myeloid cells and PBMCs from cisplatin toxicity. Also, cisplatin-induced apoptosis in HL-60 cells was rescued through reservation of mitochondrial function, inhibition of cytochrome c release to cytosol, and suppression of caspase and PARP activation. Intriguingly, cotreatment of CSE attenuated cisplatin-evoked hypocellularity of bone marrow in mice. Furthermore, we observed the enhancement of CSF-GM activity in bone marrow and spleen in mice administered CSE and cisplatin, along with increased CD11b levels in spleen. In conclusion, we uncovered a novel mechanism of CSE on myeloprotection, whereby potentially supports the use of CSE as a chemoprotector against cisplatin-induced bone marrow toxicity. Further clinical investigation of CSE in combination with cisplatin is warranted.<\/jats:p>","DOI":"10.1155\/2020\/7353618","type":"journal-article","created":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T18:31:08Z","timestamp":1579977068000},"page":"1-14","source":"Crossref","is-referenced-by-count":11,"title":["<i>Chlorella sorokiniana<\/i> Extract Prevents Cisplatin-Induced Myelotoxicity <i>In Vitro<\/i> and <i>In Vivo<\/i>"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5290-9265","authenticated-orcid":true,"given":"Shyh-Horng","family":"Lin","sequence":"first","affiliation":[{"name":"Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu 2nd Road, Hsinchu 30011, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Han","family":"Li","sequence":"additional","affiliation":[{"name":"Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu 2nd Road, Hsinchu 30011, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-4198","authenticated-orcid":true,"given":"Kai-An","family":"Chuang","sequence":"additional","affiliation":[{"name":"Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu 2nd Road, Hsinchu 30011, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4524-3615","authenticated-orcid":true,"given":"Ni-Hsuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, College of Life Sciences, National Tsing Hua University, 101 Kuang Fu 2nd Road, Hsinchu 30013, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1827-6940","authenticated-orcid":true,"given":"Chih-Hsuan","family":"Chang","sequence":"additional","affiliation":[{"name":"Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu 2nd Road, Hsinchu 30011, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2000-4127","authenticated-orcid":true,"given":"Hsin-Chieh","family":"Wu","sequence":"additional","affiliation":[{"name":"Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu 2nd Road, Hsinchu 30011, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8283-6589","authenticated-orcid":true,"given":"Ya-Hsuan","family":"Chao","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Science, National Chung-Hsing University, 145 Xingda Road, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1464-0231","authenticated-orcid":true,"given":"Chi-Chien","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Science, National Chung-Hsing University, 145 Xingda Road, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9959-6224","authenticated-orcid":true,"given":"I-Hong","family":"Pan","sequence":"additional","affiliation":[{"name":"Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu 2nd Road, Hsinchu 30011, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1884-3714","authenticated-orcid":true,"given":"Ming-Der","family":"Perng","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, College of Life Sciences, National Tsing Hua University, 101 Kuang Fu 2nd Road, Hsinchu 30013, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9066-553X","authenticated-orcid":true,"given":"Shu-Fang","family":"Wen","sequence":"additional","affiliation":[{"name":"Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 321 Kuang Fu 2nd Road, Hsinchu 30011, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1529"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2167"},{"key":"3","year":"2008"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.2174\/092986708786242903"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2011.26"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1593\/neo.08466"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdm525"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2016.04.005"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.2001.0860"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2008.10507191"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200161050-00012"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.30.2.183"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.9.3179"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(83)90075-9"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1021\/jf902952z"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0804481"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s002620050448"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(03)00082-1"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1021\/jf100369h"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-3109(96)00150-6"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/735396"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-1611-9"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot080002"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/5414297"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/s0165-2478(98)00013-3"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.21769\/bioprotoc.1631"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.108"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6290"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/s1525-0016(16)34764-5"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.961041227"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1546-9"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.4137\/BMI.S39445"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1299.025"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.18.3.511"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.03.008"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20033"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1966.28"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5754"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81334-3"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1038\/376037a0"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.33.22932"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.428"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/s0079-6603(01)67026-0"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1037"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2010.11.017"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-0273-9"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2434"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.02.001"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-015-1520-y"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2017.05.010"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081162"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1658508"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.20.19.7146-7159.2000"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414165200"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1038\/35019501"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.16.4310"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3789"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.550"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M906383199"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.2174\/157339406777934717"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1007\/bf00684843"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.3892\/or.2013.2279"},{"key":"63","first-page":"95","volume-title":"Cyclic and chronic neutropenia","year":"2008"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1016\/s0006-291x(02)02335-5"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2015.04.018"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2004.03.006"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2008.03.002"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.20.7134"},{"key":"69","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.1.271.271"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7353618.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7353618.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7353618.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T18:31:11Z","timestamp":1579977071000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/omcl\/2020\/7353618\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,25]]},"references-count":69,"alternative-id":["7353618","7353618"],"URL":"https:\/\/doi.org\/10.1155\/2020\/7353618","relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,25]]}}}