{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:38:33Z","timestamp":1747190313035,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Health Research and Development, Ministry of Health (Balitbangkes Kemenkes) of the Republic of Indonesia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2020,8,7]]},"abstract":"<jats:p><jats:italic>Cassia siamea<\/jats:italic> leaf has been proven <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> to have a strong antimalarial activity with Cassiarin A as its active compound. To obtain a source of <jats:italic>C. siamea<\/jats:italic> medicinal plant with high level of active antimalarial compound (Cassiarin A), a valid method for determining Cassiarin A level is needed. For this reason, this research conducts the validation of the Cassiarin A content with determination method using thin-layer chromatography (TLC) densitometry which includes the determination of selectivity (<jats:italic>R<\/jats:italic>s), linearity (r), accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). Cassiarin A was chromatographed on silica gel 60 F<jats:sub>254<\/jats:sub> TLC plate using chloroform\u2009:\u2009ethanol (85\u2009:\u200915 v\/v) as a mobile phase. Cassiarin A was quantified by densitometric analysis at 368\u2009nm. The linear regression analysis data for the calibration plots showed good linear relationship with <jats:italic>r<\/jats:italic>\u2009=\u20090.9995. The method was validated for precision, recovery, repeatability. The minimum detectable amount was found to be 0.0027\u2009<jats:italic>\u03bc<\/jats:italic>g\/spot, whereas the limit of quantitation was found to be 0.008\u2009<jats:italic>\u03bc<\/jats:italic>g\/spot. The results of this validation are then used to determine the Cassiarin A level of <jats:italic>C. siamea<\/jats:italic> leaf from various regions in Indonesia. Based on the results of the study, it can be concluded that the TLC-densitometry method can be used to determine level of the Cassiarin A compound with the advantages of being fast, easy, accurate, and inexpensive. In addition, it showed that <jats:italic>C. siamea<\/jats:italic> leaves from Pacitan have the highest level of Cassiarin A compared to other areas studied.<\/jats:p>","DOI":"10.1155\/2020\/7367836","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T23:44:43Z","timestamp":1596843883000},"page":"1-7","source":"Crossref","is-referenced-by-count":5,"title":["Determination of Cassiarin A Level of <i>Cassia siamea<\/i> Leaf Obtained from Various Regions in Indonesia Using the TLC-Densitometry Method"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3163-5829","authenticated-orcid":true,"given":"Wiwied","family":"Ekasari","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60115, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7410-6380","authenticated-orcid":true,"given":"Yuli","family":"Widiyastuti","sequence":"additional","affiliation":[{"name":"Medicinal Plant and Traditional Medicine Research and Development Center, National Institute Health Research and Development, Ministry of Health, Karanganyar 57792, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1596-5625","authenticated-orcid":true,"given":"Dyah","family":"Subositi","sequence":"additional","affiliation":[{"name":"Medicinal Plant and Traditional Medicine Research and Development Center, National Institute Health Research and Development, Ministry of Health, Karanganyar 57792, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6985-2071","authenticated-orcid":true,"given":"Rini","family":"Hamsidi","sequence":"additional","affiliation":[{"name":"Department of Health, Faculty of Vocational Studies, Universitas Airlangga, Surabaya 60115, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3438-7541","authenticated-orcid":true,"given":"Aty","family":"Widyawaruyanti","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60115, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5882-4251","authenticated-orcid":true,"given":"Sukmawati","family":"Basuki","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya 60131, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8009-6054","authenticated-orcid":true,"given":"Dwi","family":"Setyawan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60115, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"year":"2014","key":"1"},{"doi-asserted-by":"publisher","key":"2","DOI":"10.3987\/com-09-11680"},{"issue":"2","key":"3","first-page":"48","volume":"2","year":"2015","journal-title":"Jurnal Farmasi Dan Ilmu Kefarmasian"},{"doi-asserted-by":"publisher","key":"4","DOI":"10.21010\/ajid.v12i1s.16"},{"doi-asserted-by":"publisher","key":"5","DOI":"10.1021\/ol701623n"},{"doi-asserted-by":"publisher","key":"6","DOI":"10.1186\/1749-8546-3-7"},{"issue":"2","key":"7","doi-asserted-by":"crossref","first-page":"101","DOI":"10.2478\/v10279-012-0012-8","volume":"18","year":"2012","journal-title":"Botanica Lithuanica"},{"issue":"11","key":"8","first-page":"1266","volume":"6","year":"2007","journal-title":"African Journal of Biotechnology"},{"issue":"7","key":"9","first-page":"1201","volume":"6","year":"2012","journal-title":"Journal of Medicinal Plants Research"},{"issue":"1","key":"10","first-page":"155","volume":"4","year":"2013","journal-title":"Asian Journal of Experimental Biological Sciences"},{"volume":"6","year":"2016","journal-title":"Scientific Reports","key":"11"},{"issue":"4","key":"12","doi-asserted-by":"crossref","first-page":"762","DOI":"10.3390\/molecules23040762","volume":"23","year":"2018","journal-title":"Molecules"},{"doi-asserted-by":"publisher","key":"13","DOI":"10.31080\/asps.2019.03.0338"},{"doi-asserted-by":"publisher","key":"14","DOI":"10.22270\/jddt.v9i6-s.3784"},{"doi-asserted-by":"publisher","key":"15","DOI":"10.5530\/pj.2011.26.7"},{"doi-asserted-by":"publisher","key":"16","DOI":"10.4172\/pharmaceutical-sciences.1000365"},{"doi-asserted-by":"publisher","key":"17","DOI":"10.1016\/s1875-5364(13)60069-2"},{"doi-asserted-by":"publisher","key":"18","DOI":"10.1021\/ol8004112"},{"year":"2006","key":"19"},{"doi-asserted-by":"publisher","key":"20","DOI":"10.1016\/s0099-5428(05)32009-0"},{"doi-asserted-by":"publisher","key":"21","DOI":"10.1016\/j.jpba.2012.03.034"},{"year":"2001","journal-title":"Doctoral Program, Dissertation. 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