{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:54:16Z","timestamp":1764784456310,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,29]],"date-time":"2020-08-29T00:00:00Z","timestamp":1598659200000},"content-version":"vor","delay-in-days":241,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Any bioassay to test new chemically synthesized larvicides or phytolarvicides against <jats:italic>Culicidae<\/jats:italic> and more harmful mosquito species, such as <jats:italic>Aede<\/jats:italic>s <jats:italic>aegypti<\/jats:italic> and <jats:italic>Aedes albopictus<\/jats:italic>, which specifically transmit dengue, yellow fever, chikungunya viral fevers as well as Zika virus, or <jats:italic>Anopheles gambiae<\/jats:italic>, a vector for malaria and philariasis, requires thousands of well\u2010developed larvae, preferably at the fourth instar stage. The natural morphogenetic cycle of <jats:italic>Aedes<\/jats:italic> spp., in the field or in the laboratory, may extend to 19 days at room temperature (e.g., 25\u00b0C) from the first permanent contact between viable eggs and water and the last stage of larval growth or metamorphosis into flying adults. Thus, accelerated sequential molting is desirable for swifter bioassays of larvicides. We achieved this goal in <jats:italic>Aedes aegypti<\/jats:italic> with very limited strategic and low\u2010cost additions to food, such as coconut water, milk or its casein, yeast extract, and to a lesser extent, glycerol. The naturally rich coconut water was excellent for quickly attaining the population of instar IV larvae, the most advanced one before pupation, saving about a week, for subsequent larvicidal bioassays. Diluted milk, as another food source, allowed an even faster final ecdysis and adults are useful for mosquito taxonomical purpose.<\/jats:p>","DOI":"10.1155\/2020\/7405421","type":"journal-article","created":{"date-parts":[[2020,8,29]],"date-time":"2020-08-29T23:34:19Z","timestamp":1598744059000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Accelerating the Morphogenetic Cycle of the Viral Vector <i>Aedes aegypti<\/i> Larvae for Faster Larvicidal Bioassays"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9967-7091","authenticated-orcid":false,"given":"Jos\u00e9 Domingos","family":"Fontana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Lopes","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiana","family":"Zuccolotto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cibelle","family":"de Borba Dallagassa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Pellizzari","family":"Wielewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara Maria Santano","family":"Chalcoski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Antonio Navarro","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinicius Sobrinho","family":"Richardi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas","family":"Golart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynara","family":"de Melo Rodovalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,29]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2017.59"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30518-2"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"volume-title":"Dengue 2019","year":"2020","author":"Felix P.","key":"e_1_2_10_4_2"},{"key":"e_1_2_10_5_2","unstructured":"WHOandPAHO Epidemiological update yellow fever 2017 https:\/\/reliefweb.int\/sites\/reliefweb.int\/files\/resources\/2017-dec-13-phe-epi-update-yellow-fever.pdf."},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.03.010"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-019-09647-7"},{"key":"e_1_2_10_8_2","first-page":"11","article-title":"Food as a limiting factor for Aedes aegypti in water-storage containers","volume":"29","author":"Arrivillaga J.","year":"2004","journal-title":"Journal of Vector Ecology"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1603\/033.046.0105"},{"volume-title":"Aedes aegypti: Biology and Ecology","year":"1986","author":"Nelson M. J.","key":"e_1_2_10_10_2"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.589.1.1"},{"key":"e_1_2_10_12_2","first-page":"1","article-title":"Morphology and morphometry of Aedes aegypti larvae","volume":"3","author":"Bar A.","year":"2013","journal-title":"Annual Review and Research in Biology"},{"key":"e_1_2_10_13_2","first-page":"52","article-title":"Morphology and morphometry of Aedes aegypti adult mosquito","volume":"3","author":"Bar A.","year":"2013","journal-title":"Annual Review and Research in Biology"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3409"},{"volume-title":"spss 16.0 Advanced Statistical Procedures Companion; SPSS 16.0 Guide to Data Analysis","year":"2008","author":"Norusis M. J.","key":"e_1_2_10_15_2"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151403"},{"key":"e_1_2_10_17_2","first-page":"465","article-title":"Food material and feeding procedures for mosquito larvae","volume":"31","author":"Asahina S.","year":"1964","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.3923\/ajps.2004.300.305"},{"volume-title":"Modern Milk Products","year":"1979","author":"Malcolm E. C.","key":"e_1_2_10_19_2"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.2298\/jsc0705451v"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1590\/s1516-89132003000100017"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1051\/fruits\/2012002"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14125144"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0034-89102002000200009"},{"key":"e_1_2_10_25_2","unstructured":"SanchesT. S. Molecular studies of trypsin-like enzymes present in midgut of Aedes aegypti larvae [Ph.D. Thesis] 2009 UNIFESP - Federal University of S\u00e3o Paulo."},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjy073"},{"volume-title":"Aedes aegypti (L.), The Yellow Fever Mosquito\u2014Its history, Bionomics and Structure","year":"1960","author":"Christopher S. R.","key":"e_1_2_10_27_2"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1515\/pjen-2017-0008"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.02.001"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087468"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5916.176632"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2011.00971.x"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/53.2.277"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-105"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/s41043-017-0080-6"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00099"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-391"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-012-1343-y"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.4103\/2229-5070.129167"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187420"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/7405421.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/7405421.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/7405421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T11:41:52Z","timestamp":1735645312000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/7405421"}},"subtitle":[],"editor":[{"given":"Ceferino M.","family":"L\u00f3pez S\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/7405421"],"URL":"https:\/\/doi.org\/10.1155\/2020\/7405421","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2020-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"7405421"}}