{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:41:14Z","timestamp":1781656874393,"version":"3.54.5"},"reference-count":73,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T00:00:00Z","timestamp":1593734400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970805"],"award-info":[{"award-number":["81970805"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670861"],"award-info":[{"award-number":["81670861"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970805"],"award-info":[{"award-number":["81970805"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670861"],"award-info":[{"award-number":["81670861"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2020,7,3]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. Adenosine A<jats:sub>2A<\/jats:sub> receptor (A<jats:sub>2A<\/jats:sub>R) signaling is neuroprotective in some retinal damage models, but its role in neuronal survival during retinal detachment (RD) is unclear. We tested the hypothesis that A<jats:sub>2A<\/jats:sub>R antagonist ZM241385 would prevent photoreceptor apoptosis by inhibiting retinal inflammation and oxidative stress after RD. <jats:italic>Methods<\/jats:italic>. The A<jats:sub>2A<\/jats:sub>R antagonist ZM241385 was delivered daily to C57BL\/6J mice for three days at a dose (3\u2009mg\/kg, i.p.) starting 2 hours prior to creating RD. A<jats:sub>2A<\/jats:sub>R expression, microglia proliferation and reactivity, glial fibrillary acidic protein (GFAP) accumulation, IL-1<jats:italic>\u03b2<\/jats:italic> expression, and reactive oxygen species (ROS) production were evaluated with immunofluorescence. Photoreceptor TUNEL was analyzed. <jats:italic>Results<\/jats:italic>. A<jats:sub>2A<\/jats:sub>R expression obviously increased and accumulated in microglia and M\u00fcller cells in the retinas after RD. The A<jats:sub>2A<\/jats:sub>R antagonist ZM241385 effectively inhibited retinal microglia proliferation and reactivity, decreased GFAP upregulation and proinflammatory cytokine IL-1<jats:italic>\u03b2<\/jats:italic> expression of M\u00fcller cells, and suppressed ROS overproduction, resulting in attenuation of photoreceptor apoptosis after RD. <jats:italic>Conclusions<\/jats:italic>. The A<jats:sub>2A<\/jats:sub>R antagonist ZM241385 is an effective suppressor of microglia proliferation and reactivity, gliosis, neuroinflammation, oxidative stress, and photoreceptor apoptosis in a mouse model of RD. This suggests that A<jats:sub>2A<\/jats:sub>R blockade may be an important therapeutic strategy to protect photoreceptors in RD and other CNS diseases that share a common etiology.<\/jats:p>","DOI":"10.1155\/2020\/7649080","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T23:35:50Z","timestamp":1593819350000},"page":"1-12","source":"Crossref","is-referenced-by-count":17,"title":["Blockade of Adenosine A<sub>2A<\/sub> Receptor Protects Photoreceptors after Retinal Detachment by Inhibiting Inflammation and Oxidative Stress"],"prefix":"10.1155","volume":"2020","author":[{"given":"Sha","family":"Gao","sequence":"first","affiliation":[{"name":"The Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin 2nd Road, Shanghai 200025, China"},{"name":"The Department of Ophthalmology, Ruijin North Hospital, Shanghai Jiao Tong University School of Medicine, 999, Xiwang Road, Shanghai 201801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"The Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin 2nd Road, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanuo","family":"Wang","sequence":"additional","affiliation":[{"name":"The Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin 2nd Road, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5604-6591","authenticated-orcid":true,"given":"Yisheng","family":"Zhong","sequence":"additional","affiliation":[{"name":"The Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin 2nd Road, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9669-6521","authenticated-orcid":true,"given":"Xi","family":"Shen","sequence":"additional","affiliation":[{"name":"The Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin 2nd Road, Shanghai 200025, China"},{"name":"The Department of Ophthalmology, Ruijin North Hospital, Shanghai Jiao Tong University School of Medicine, 999, Xiwang Road, Shanghai 201801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.120.11.1435"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.119.4.499"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2425"},{"issue":"6","key":"4","first-page":"990","volume":"36","year":"1995","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2004.11.046"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-24489"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0897-18.2018"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608167104"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4421"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22752"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2006.05.003"},{"key":"12","first-page":"417","volume":"21","year":"2015","journal-title":"Molecular Vision"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17400-3"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14565"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1209"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0901"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2011.03.007"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-019-09667-0"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1996.0352"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2807-12.2013"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1354-05.2005"},{"issue":"1","key":"22","first-page":"230","volume":"41","year":"2000","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2003.tb00475.x"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.451"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2638"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03395.x"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1097\/nen.0b013e3180517477"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2010.12.001"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1021\/cn5001606"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801022-8.00006-4"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20733-2"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23579"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-22945"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.3791\/50660"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.05.006"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2015.02.020"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1038\/srep42846"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8640284"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23336"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.73"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-18024"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.34211"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.7150\/thno.28778"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.24.3.161.8305"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-0827"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-53627-y"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2018.09.053"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0607359"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2005.04.004"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-018-0038-1"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2009.08.002"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/297241"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0b013e3182934aca"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/j.nurt.2010.05.014"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1159\/000443893"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-005-0649-1"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/673090"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0333-5"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1002\/path.1645"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2013.12.071"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(03)30005-1"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-1398"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-23-08693.2000"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0000000000000200"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.1104"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2016.08.004"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3955-14.2015"},{"key":"68","first-page":"867","volume":"12","year":"2006","journal-title":"Molecular Vision"},{"key":"69","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.286"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0598"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1009179107"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6797"},{"key":"73","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.12.002"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7649080.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7649080.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2020\/7649080.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T23:35:55Z","timestamp":1593819355000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/omcl\/2020\/7649080\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,3]]},"references-count":73,"alternative-id":["7649080","7649080"],"URL":"https:\/\/doi.org\/10.1155\/2020\/7649080","relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,3]]}}}