{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:09:35Z","timestamp":1790053775528,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T00:00:00Z","timestamp":1592870400000},"content-version":"vor","delay-in-days":174,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010227","name":"Medical Science and Technology Development Foundation, Nanjing Municipality Health Bureau","doi-asserted-by":"publisher","award":["YKK18193"],"award-info":[{"award-number":["YKK18193"]}],"id":[{"id":"10.13039\/501100010227","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010227","name":"Medical Science and Technology Development Foundation, Nanjing Municipality Health Bureau","doi-asserted-by":"publisher","award":["YKK17213"],"award-info":[{"award-number":["YKK17213"]}],"id":[{"id":"10.13039\/501100010227","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970664"],"award-info":[{"award-number":["81970664"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670650"],"award-info":[{"award-number":["81670650"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Idiopathic nephrotic syndrome (INS) is a disease involving injury to podocytes in the glomerular filtration barrier, and its specific causes have not been elucidated. Transfer RNA\u2010derived fragments (tRFs), products of precise tRNA cleavage, have been indicated to play critical roles in various diseases. Currently, there is no relevant research on the role of tRFs in INS. This study intends to explore the changes in and importance of tRFs during podocyte injury in vitro and to further analyze the potential mechanism of INS. Differentially expressed tRFs in the adriamycin\u2010treated group were identified by high\u2010throughput sequencing and further verified by quantitative RT\u2010PCR. In total, 203 tRFs with significant differential expression were identified, namely, 102 upregulated tRFs and 101 downregulated tRFs (<jats:italic>q<\/jats:italic> &lt; 0.05, \u2223log2FC | \u22652). In particular, AS\u2010tDR\u2010008924, AS\u2010tDR\u2010011690, tDR\u2010003634, AS\u2010tDR\u2010013354, tDR\u2010011031, AS\u2010tDR\u2010001008, and AS\u2010tDR\u2010007319 were predicted to be involved in podocyte injury by targeting the Gpr, Wnt, Rac1, and other genes. Furthermore, gene ontology analysis showed that these differential tRFs were strongly associated with podocyte injury processes such as protein binding, cell adhesion, synapses, the actin cytoskeleton, and insulin\u2010activate receptor activity. KEGG pathway analysis predicted that they participated in the PI3K\u2010Akt signaling pathway, Wnt signaling pathway, and Ras signaling pathway. It was reported that these pathways contribute to podocyte injury. In conclusion, our study revealed that changes in the expression levels of tRFs might be involved in INS. Seven of the differentially expressed tRFs might play important roles in the process of podocyte injury and are worthy of further study.<\/jats:p>","DOI":"10.1155\/2020\/7826763","type":"journal-article","created":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T23:34:26Z","timestamp":1592955266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["tRNA\u2010Derived Fragments in Podocytes with Adriamycin\u2010Induced Injury Reveal the Potential Mechanism of Idiopathic Nephrotic 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