{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T18:12:12Z","timestamp":1772820732735,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T00:00:00Z","timestamp":1598227200000},"content-version":"vor","delay-in-days":236,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560335"],"award-info":[{"award-number":["81560335"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Cystic echinococcosis<\/jats:italic> (CE) is a zoonotic disease caused by <jats:italic>Echinococcus granulosus<\/jats:italic> (Eg) infection. Our previous study confirmed that recombinant Eg.P29 (rEg.P29) could protect against <jats:italic>echinococcus granulosus<\/jats:italic> secondary infection in sheep and mice. The aim of the study was to investigate the association between immunoprotection of rEg.P29 vaccine and mmu\u2010miR\u2010374b\u20105p (miR\u2010374b\u20105p) and study the immunity influence of miR\u2010374b\u20105p on CD4<jats:sup>+<\/jats:sup> T cells in mice spleen. MiR\u2010374b\u20105p level was significantly increased after the second\u2010week and the fourth week of vaccination with rEg.P29. Overexpression of miR\u2010374b\u20105p increased IFN\u2010<jats:italic>\u03b3<\/jats:italic>, IL\u20102, IL\u201017A mRNA levels and decreased IL\u201010 mRNA levels in CD4<jats:sup>+<\/jats:sup> T cells. Moreover, the inhibition of miR\u2010374b\u20105p decreased IFN\u2010<jats:italic>\u03b3<\/jats:italic> and IL\u201017A and increased IL\u201010 mRNA levels in CD4<jats:sup>+<\/jats:sup> T cells; this was further confirmed by the flow cytometry. The vaccination of rEg.P29 enhanced miR\u2010374b\u20105p expression that was associated with a higher Th1 and Th17 immune response, a lower IL\u201010 mRNA production with miR\u2010374b\u20105p overexpression, a lower Th1 immune response, and a higher IL\u201010 mRNA levels with miR\u2010374b\u20105p inhibitions. To sum up, these data suggest that miR\u2010374b\u20105p may participate in rEg.P29 immunity by regulating Th1 and Th17 differentiation.<\/jats:p>","DOI":"10.1155\/2020\/8024763","type":"journal-article","created":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T23:32:27Z","timestamp":1598311947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["MiR\u2010374b\u20105p Regulates T Cell Differentiation and Is Associated with rEg.P29 Immunity"],"prefix":"10.1155","volume":"2020","author":[{"given":"Dongjie","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiancai","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingxing","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songhao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7744-115X","authenticated-orcid":false,"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,24]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00075-18"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldx033"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12649"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12282"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.01.002"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003795"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.029"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1684\/ecn.2012.0314"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2263-9"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12301"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0229121"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098357"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-016-9656-7"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmn009"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007932"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03012"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.04.011"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02148"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20150990"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2018.07.005"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12406"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14219"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2018.4315"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.7951"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20181436"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184631"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-0832-7"},{"key":"e_1_2_8_28_2","article-title":"microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach","volume":"8","author":"Macchiaroli N.","year":"2015","journal-title":"Parasites &Vectors"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007032"},{"key":"e_1_2_8_30_2","article-title":"Differentiation of pathogenic Th17 cells is negatively regulated by Let-7 microRNAs in a mouse model of multiple sclerosis","volume":"10","author":"Angelou C. 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