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Around 280,000 people die from it annually, and more than 779 million people are at risk of getting infected. The schistosome species which infect human beings are <jats:italic>Schistosoma mansoni<\/jats:italic>, <jats:italic>Schistosoma haematobium<\/jats:italic>, <jats:italic>Schistosoma intercalatum<\/jats:italic>, <jats:italic>Schistosoma japonicum<\/jats:italic>, <jats:italic>Schistosoma guineensis<\/jats:italic>, and <jats:italic>Schistosoma mekongi<\/jats:italic>. This disease is also of veterinary significance; the most important species being <jats:italic>Schistosoma bovis<\/jats:italic> since it causes the disease in around 160 million livestock in Africa and Asia. This work was aimed at designing and developing a genus-specific loop-mediated isothermal amplification (LAMP) method for detecting the most important schistosome species affecting humans and for the species-specific detection of <jats:italic>S. bovis<\/jats:italic>. Bioinformatics tools were used for primer design, and the LAMP method was standardised for detecting the ITS-1 region from <jats:italic>S. intercalatum<\/jats:italic>, <jats:italic>S. haematobium<\/jats:italic>, <jats:italic>S. mansoni<\/jats:italic>, <jats:italic>S. japonicum<\/jats:italic>, and <jats:italic>S. bovis<\/jats:italic> DNA (generic test) and the NADH 1 gene for specifically detecting <jats:italic>S. bovis<\/jats:italic> (at different DNA concentrations). Detection limits achieved were 1\u2009pg DNA for <jats:italic>S. mansoni<\/jats:italic>, 0.1\u2009pg for <jats:italic>S. haematobium<\/jats:italic>, 1\u2009pg for <jats:italic>S. intercalatum<\/jats:italic>, and 10\u2009pg for <jats:italic>S. bovis<\/jats:italic>. No amplification for <jats:italic>S. japonicum<\/jats:italic> DNA was obtained. The LAMP designed for the amplification of <jats:italic>S. bovis<\/jats:italic> NADH-1 worked specifically for this species, and no other DNA from other schistosome species included in the study was amplified. Two highly sensitive LAMP methods for detecting different <jats:italic>Schistosoma<\/jats:italic> species important for human and veterinary health were standardised. These methods could be very useful for the diagnosis and surveillance of schistosome infections.<\/jats:p>","DOI":"10.1155\/2020\/8042705","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T23:38:40Z","timestamp":1595633920000},"page":"1-11","source":"Crossref","is-referenced-by-count":10,"title":["Molecular Markers for Detecting <i>Schistosoma<\/i> Species by Loop-Mediated Isothermal Amplification"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4089-4307","authenticated-orcid":true,"given":"Pedro","family":"Fern\u00e1ndez-Soto","sequence":"first","affiliation":[{"name":"Infectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca, Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, 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