{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:32:55Z","timestamp":1780986775562,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"vor","delay-in-days":147,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471409"],"award-info":[{"award-number":["81471409"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. Studies have shown that corneas of young children were more susceptible to Ultraviolet B (UVB) radiation damage. However, there exist limited information about the harm of UVB to eyes and preventive measures on infancy. Vitamin C as an antioxidant is widely used to prevent many diseases. Therefore, the aim of this study was to explore the protective effect of vitamin C on the cornea of infant rats with acute UVB injury. <jats:italic>Method<\/jats:italic>. Thirty\u2010six infant rats were randomly divided into three groups: control (CON) group, UVB (UVB) group, and UVB+vitamin C (UVB+VitC) group. The UVB group was exposed to UVB irradiation (8\u2009J\/cm<jats:sup>2<\/jats:sup>, 15\u2009min\/d, 7\u2009d) and the UVB+vitamin C group suffered the same UVB irradiation treated with vitamin C at the dose of 40\u2009mg\/kg via intraperitoneal injection. Then, corneal morphology was detected <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> at 7\u2009d post\u2010UVB exposure. Furthermore, serum inflammatory factors (IL\u20101, IL\u20106, and TNF\u2010<jats:italic>\u03b1<\/jats:italic>) and oxidative status (4\u2010HNE and MDA) were detected by ELISA, and the expression of vascular endothelial growth factor\u2010<jats:italic>\u03b1<\/jats:italic> (VEGF\u2010<jats:italic>\u03b1<\/jats:italic>) and superoxide dismutase (SOD) in the cornea was detected by western blot or immunofluorescent staining. <jats:italic>Results<\/jats:italic>. Slit lamp detection revealed that the area of corneal desquamation and corneal neovascularization in the UVB+VitC group was significantly less than those in the UVB group at 7\u2009d post\u2010UVB exposure (all <jats:italic>p<\/jats:italic> &lt; 0.05). OCT results showed that the thickness of the central cornea in the UVB+VitC group was decreased than that in the UVB group (<jats:italic>p<\/jats:italic> &lt; 0.05). The serum inflammatory factors (IL\u20101, IL\u20106, and TNF\u2010<jats:italic>\u03b1<\/jats:italic>) and oxidative status (4\u2010HNE and MDA) in the UVB group were significantly increased compared with the CON group (all <jats:italic>p<\/jats:italic> &lt; 0.05), while those factors in the UVB+VitC group were decreased compared with those in the UVB group. Furthermore, the expression of VEGF\u2010<jats:italic>\u03b1<\/jats:italic> in the UVB+VitC group was dramatically decreased compared with that in the UVB group (<jats:italic>p<\/jats:italic> &lt; 0.05), and the expression of SOD2 in the UVB+VitC group was dramatically increased compared with that in the UVB group at 7\u2009d post\u2010UVB exposure (<jats:italic>p<\/jats:italic> &lt; 0.05). <jats:italic>Conclusion<\/jats:italic>. Vitamin C could protect infant rats from corneal injury induced by UVB via alleviating corneal edema, improving corneal inflammatory reaction, and decreasing VEGF\u2010<jats:italic>\u03b1<\/jats:italic> expression.<\/jats:p>","DOI":"10.1155\/2020\/8089273","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T23:39:24Z","timestamp":1590622764000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Protective Effect of Vitamin C against Infancy Rat Corneal Injury Caused by Acute UVB 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