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The study is aimed at investigating the therapeutic effect of Zhuang\u2010Gu\u2010Fang in ovariectomized rats. Fifty six\u2010month\u2010old Wistar rats were randomly selected and divided into 5 groups (<jats:italic>n<\/jats:italic>\u2009=\u200910), namely, model group, positive group, low\u2010dose Chinese medicine group, medium\u2010dose group, and high\u2010dose group. Another 10 sham operation Wistar rats were taken as a negative control group. After 3 months of intervention, the bone mineral density (BMD), procollagen type I N\u2010peptide (PINP), beta C\u2010terminal cross\u2010linked telopeptides of type I collagen carboxyl\u2010terminal peptide (<jats:italic>\u03b2<\/jats:italic>\u2010CTX), Leptin, Ghrelin, and Peptide YY (PYY) of each group were measured. Besides, the ultrastructure of bone structure and osteoblasts was also observed by transmission electron microscopy. Western blot method was used to detect the expression levels of Leptin and Ghrelin in bone tissue, and RT\u2010PCR detected the mRNA expression levels of Leptin and Ghrelin. BMD test indicated that Zhuang\u2010Gu\u2010Fang could effectively prevent the loss of tibia bone in ovariectomized rats. Histomorphology analysis showed that Zhuang\u2010Gu\u2010Fang could preserve trabecular bone structure integrity and improve osteoblast ultrastructure. Notably, the study found out that Zhuang\u2010Gu\u2010Fang worked through balancing the bone metabolism via increasing bone formation\/resorption ratio. Additionally, Zhuang\u2010Gu\u2010Fang highlighted the recovery effects in multiple levels of osteogenesis\u2010 and osteanagenesis\u2010related factors Leptin, Ghrelin, and PYY. Conclusively, the study proved the therapeutic potential of the Zhuang\u2010Gu\u2010Fang for postmenopausal osteoporosis (PMOP) and further revealed that its therapeutic effect was related to the balance of bone metabolism and the recovery effects of bone\u2010related factors Leptin, Ghrelin, and PYY.<\/jats:p>","DOI":"10.1155\/2020\/8164064","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T04:53:30Z","timestamp":1605675210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Zhuang\u2010Gu\u2010Fang Treats Osteoporosis in Ovariectomized Rats by Increasing the Osteogenesis\u2010Related Factors Leptin, Ghrelin, and 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