{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:21:40Z","timestamp":1767165700412,"version":"build-2238731810"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ningbo Social and Scientific Development Fund","award":["2015C50012"],"award-info":[{"award-number":["2015C50012"]}]},{"name":"Ningbo Social and Scientific Development Fund","award":["2017A610250"],"award-info":[{"award-number":["2017A610250"]}]},{"name":"Ningbo Social and Scientific Development Fund","award":["2015XYY15040"],"award-info":[{"award-number":["2015XYY15040"]}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2015C50012"],"award-info":[{"award-number":["2015C50012"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2017A610250"],"award-info":[{"award-number":["2017A610250"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2015XYY15040"],"award-info":[{"award-number":["2015XYY15040"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ningbo University\u2019s Scientific Research Project","award":["2015C50012"],"award-info":[{"award-number":["2015C50012"]}]},{"name":"Ningbo University\u2019s Scientific Research Project","award":["2017A610250"],"award-info":[{"award-number":["2017A610250"]}]},{"name":"Ningbo University\u2019s Scientific Research Project","award":["2015XYY15040"],"award-info":[{"award-number":["2015XYY15040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Respiratory Journal"],"published-print":{"date-parts":[[2020,3,31]]},"abstract":"<jats:p>\n                    In recent years, various biomarkers have been gradually applied on bronchoalveolar lavage (BAL) fluid for the diagnosis of invasive pulmonary aspergillosis (IPA). The objective of this study is to assess the value of the liquid-based cytopathology test (LCT) for improving the identification of IPA in BAL fluid from possible IPA patients, following special staining with periodic acid-Schiff staining (PAS) or Grocott\u2019s methenamine silver (GMS). A total of 47 consecutive possible IPA patients who underwent bronchoscopy with BAL fluid from January 2017 to December 2018 were included. 45 people had a pair of BAL fluid specimens and 2 patients had two BAL fluid specimens. The 49 pairs of BAL fluid specimens were processed for culture, tuberculosis acid fast staining smear, direct microbial smear, and LCT with special staining (PAS and GMS), respectively. Then, we compared the sensitivity and specificity of PAS and GMS in BAL fluid in high-risk patients. Among 47 possible IPA patients, 25 patients had proven\/probable IPA, and 11 patients had other invasive fungal diseases. The sensitivity of GMS was higher than that of PAS (92.11% versus 81.58%;\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mrow>\n                        <mml:mi>P<\/mml:mi>\n                        <mml:mo>=<\/mml:mo>\n                        <mml:mn>0.175<\/mml:mn>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ). The specificity of GMS was 81.82%, which was higher than that of PAS (81.82% versus 72.73%;\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                      <mml:mrow>\n                        <mml:mi>P<\/mml:mi>\n                        <mml:mo>=<\/mml:mo>\n                        <mml:mn>0.611<\/mml:mn>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ). The negative predictive value (NPV) for PAS and GMS were 53.33% and 75.00%, respectively. The positive predictive value (PPV) for PAS and GMS were 91.18% and 94.59%, respectively. This study showed that special staining of LCT in BAL fluid may be a novel method for the diagnosis of IPA, and the GMS of LCT had higher sensitivity and specificity, which was superior to PAS.\n                  <\/jats:p>","DOI":"10.1155\/2020\/8243473","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T19:51:29Z","timestamp":1585684289000},"page":"1-9","source":"Crossref","is-referenced-by-count":4,"title":["Special Staining of the Liquid-Based Cytopathology Test in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis with Nonneutropenic Patients"],"prefix":"10.1155","volume":"2020","author":[{"given":"Yue","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deng","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Cell, Clinicopathological Diagnosis Center of Ningbo, 685 Huancheng North Road, Ningbo, Zhejiang 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Cell, Clinicopathological Diagnosis Center of Ningbo, 685 Huancheng North Road, Ningbo, Zhejiang 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianfa","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cell, Clinicopathological Diagnosis Center of Ningbo, 685 Huancheng North Road, Ningbo, Zhejiang 315211, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiming","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5585-0980","authenticated-orcid":true,"given":"Qidong","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4562-2124","authenticated-orcid":true,"given":"Zaichun","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7082-1750","authenticated-orcid":true,"given":"Zhongbo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, Zhejiang 315020, 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