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Albendazole (ABZ) and mebendazole are effective against CE, but a high dosage in a long-term period is usually required. In this study, we evaluate the effects of DNA damage repair inhibitor (i.e., Veliparib) in combination with artesunate (AS) on hydatid cysts. For the <jats:italic>in vitro<\/jats:italic> assay, protoscoleces of <jats:italic>E. granulosus<\/jats:italic> (<jats:italic>E.g<\/jats:italic> PSCs) were incubated with low AS (AS-L, 65\u2009<jats:italic>\u03bc<\/jats:italic>M), moderate AS (AS-M, 130\u2009<jats:italic>\u03bc<\/jats:italic>M), and high AS (AS-H, 325\u2009<jats:italic>\u03bc<\/jats:italic>M), AS-L\/M\/H+Veliparib (10\u2009<jats:italic>\u03bc<\/jats:italic>M), and ABZ (25\u2009<jats:italic>\u03bc<\/jats:italic>M), respectively. The AS-H+Veliparib group showed the maximal protoscolicidal effects. Ultrastructural change revealed that germinal layer (GL) cells were reduced, and lipid droplets appeared. AS could induce DNA injuries in PSCs. The 8-OHdG was expressed in the PSCs and GL of the cysts in mice, especially in the presence of Veliparib. The most severe DNA damages were observed in the AS-H+Veliparib group. Meanwhile, the expression of ribosomal protein S9 (RPS9) gene in the AS-H+Veliparib group was significantly lower than that in the AS-H group. The <jats:italic>in vivo<\/jats:italic> chemotherapeutic effects of AS-L (50\u2009mg\/kg), AS-H (200\u2009mg\/kg), and AS-H+Veliparib (25\u2009mg\/kg) were assessed in experimentally infected mice. Upon 6 weeks of oral administration, ultrasonography was used to monitor the volume change of vesicles. Maximum potentiation was seen on day 15 with values (versus AS) of 34 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>) for AS-H\u2009+\u2009Veliparib. It led to the reduction of cyst weight (55.40%) compared with the model group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>), which was better than AS alone (52.84%) and ABZ-treated mice (55.35%). Analysis of cysts collected from AS-H+Veliparib-treated mice by transmission electron microscopy revealed a drug-induced structural destruction. The structural integrity of the germinal layer was lost, and the majority of the microtriches disappeared. In conclusion, our study demonstrates that AS or AS in combination with Veliparib is effective for treating CE, especially the combination group. On this basis, AS represented promising drug candidates in anti-CE chemotherapy.<\/jats:p>","DOI":"10.1155\/2020\/8259820","type":"journal-article","created":{"date-parts":[[2020,7,4]],"date-time":"2020-07-04T23:32:01Z","timestamp":1593905521000},"page":"1-11","source":"Crossref","is-referenced-by-count":4,"title":["In Vitro and In Vivo Efficacy of DNA Damage Repair Inhibitor Veliparib in Combination with Artesunate against <i>Echinococcus granulosus<\/i>"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7463-8021","authenticated-orcid":true,"given":"Y. F.","family":"Li","sequence":"first","affiliation":[{"name":"Pharmaceutical Department, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"},{"name":"College of Pharmaceutical Sciences, Xinjiang Medical University, Urumqi 830054, China"},{"name":"State Key Laboratory of Pathogenesis, Prevention, Treatment of Central Asian High Incidence Diseases, Urumqi 830054, China"},{"name":"Clinical Pharmacy, The Third Affiliated Hospital of Bengbu Medical College, Suzhou 234000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3714-4107","authenticated-orcid":true,"given":"L. M.","family":"Wen","sequence":"additional","affiliation":[{"name":"Pharmaceutical Department, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"},{"name":"College of Pharmaceutical Sciences, Xinjiang Medical University, Urumqi 830054, China"},{"name":"State Key Laboratory of Pathogenesis, Prevention, Treatment of Central Asian High Incidence Diseases, Urumqi 830054, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Pharmaceutical Department, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China"},{"name":"State Key Laboratory of Pathogenesis, Prevention, Treatment of Central Asian High Incidence Diseases, Urumqi 830054, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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