{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:39:24Z","timestamp":1789684764049,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012964","name":"Minist\u00e8re de l\u2019Enseignement Sup\u00e9rieur et de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012964","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2020,3,31]]},"abstract":"<jats:p>This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of <jats:italic>Punica granatum<\/jats:italic> leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against <jats:italic>Staphylococcus aureus<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic> standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and <jats:italic>P. granatum<\/jats:italic> leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant <jats:italic>E. coli<\/jats:italic> and penicillin-resistant <jats:italic>S. aureus<\/jats:italic>. These results can be assigned to tannins, flavonoids, and phenolic acids found in <jats:italic>P. granatum<\/jats:italic> leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.<\/jats:p>","DOI":"10.1155\/2020\/8271203","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T23:54:22Z","timestamp":1585698862000},"page":"1-7","source":"Crossref","is-referenced-by-count":59,"title":["Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of <i>Punica granatum<\/i> (Pomegranate) Leaves"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1852-5265","authenticated-orcid":true,"given":"Amine","family":"Trabelsi","sequence":"first","affiliation":[{"name":"Pharmacognosy Laboratory, Faculty of Pharmacy, University of Monastir, Avicenna Street, Monastir 5000, Tunisia"},{"name":"Research Unit of Bioactive and Natural Substances and Biotechnology UR17 ES49, Faculty of Dental Medicine, University of Monastir, Avicenna Street, Monastir 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